Tsipas George, Tsiamis George, Vidalis Kosmas, Bourtzis Kostas
Department of Environmental and Natural Resources Management, University of Ioannina, Agrinio, Greece.
Genetica. 2009 Jul;136(3):491-500. doi: 10.1007/s10709-008-9331-1. Epub 2008 Nov 13.
The genetic structure of the Western Greece lake populations of Carassius gibelio and Cyprinus carpio carpio populations was characterized by using a PCR-based RFLP and sequencing analysis of mitochondrial rDNA genes and regions (16S rDNA, cytochrome b and D-loop). Our analysis was able to detect: (a) two haplotypes in C. c. carpio populations and two haplotypes in C. gibelio populations (b) a high nucleotide divergence between the two species and (c) two genetically distinct C. gibelio populations, one existing in the Amvrakia habitat (AMV1) with a second in Ozeros and Trichonida (OZE1 and TRI1) habitat. The present analysis indicates that genetic diversity observed was limited with a haplotype index between 0.0 and 55.6%, and a nucleotide diversity within and among populations between 0.0 and 1.27%. It also underlines a restricted mtDNA-based evaluation of the phylogenetic relationships among C. gibelio and C. c. carpio populations. In addition, the present study contributed knowledge on the genetic variation and structure of these populations which is absolutely necessary for any efficient fish management and/or conservation programme.
通过基于PCR的线粒体rDNA基因及区域（16S rDNA、细胞色素b和D环）的限制性片段长度多态性（RFLP）和测序分析，对希腊西部湖泊中银鲫和鲤鱼种群的遗传结构进行了表征。我们的分析能够检测到：（a）鲤鱼种群中有两种单倍型，银鲫种群中有两种单倍型；（b）这两个物种之间存在高度的核苷酸差异；（c）两个遗传上不同的银鲫种群，一个存在于阿姆夫拉基亚栖息地（AMV1），另一个存在于奥泽罗斯和特里科尼达（OZE1和TRI1）栖息地。目前的分析表明，观察到的遗传多样性有限，单倍型指数在0.0%至55.6%之间，种群内和种群间的核苷酸多样性在0.0%至1.27%之间。它还强调了基于线粒体DNA对银鲫和鲤鱼种群之间系统发育关系的评估有限。此外，本研究提供了关于这些种群遗传变异和结构的知识，这对于任何有效的鱼类管理和/或保护计划来说都是绝对必要的。
