Rigby Neil M, Marsh Justin, Sancho Ana I, Wellner Klaus, Akkerdaas Jaap, van Ree Ronald, Knulst Andre, Fernández-Rivas Montserrat, Brettlova Vlasta, Schilte Piet P, Summer Colin, Pumphrey Richard, Shewry Peter R, Mills E N Clare
Institute of Food Research, Norwich, UK.
Mol Nutr Food Res. 2008 Nov;52 Suppl 2:S251-61. doi: 10.1002/mnfr.200800083.
A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis.
脂质转移蛋白（LTP，Cor a 8）与11S（Cor a 9）和7S种子贮藏球蛋白（Cor a 11）是榛子中存在的主要食物过敏原。本文描述了使用凝胶内胰蛋白酶消化质谱法对它们进行纯化和表征的方法，以确认其身份，并使用圆二色光谱法和傅里叶变换红外光谱法来证明它们具有正确折叠。初步免疫化学研究还证实，通过使用榛子过敏患者的血清进行免疫印迹测定，纯化后的制剂在免疫球蛋白E结合方面保留了其免疫特性。这些制剂为基于成分解析诊断概念开发改进的食物过敏诊断方法奠定了基础。
