Wackym P A, Ward P H, House W F, Linthicum F H, Micevych P E
Division of Head and Neck Surgery, UCLA Medical Center 90024-1624.
Otolaryngol Head Neck Surg. 1991 Jan;104(1):72-80. doi: 10.1177/019459989110400114.
A surgical technique is presented to obtain the entire vestibular aqueduct, containing the endolymphatic duct and sac as well as the vestibular epithelia, from the maculae and cristae during labyrinthectomy. The inner ear tissue was fixed in 3% glutaraldehyde, decalcified in 0.1 mol/L Na-EDTA, and routinely processed for transmission electron microscopy, including post-fixation with osmium tetroxide. Postembedding protein A-colloidal gold immunoelectron microscopy was performed after exposure of cellular antigenic sites by sodium metaperiodate. To validate this technique, thin sections from the crista ampullaris and the endolymphatic sac were incubated with antibodies raised against S-100, a protein present in specific types of neural tissue. Specific immunoreactivity was observed in the crista ampullaris, but not in the endolymphatic sac. The surgical biopsy technique described provides a means for the neuro-otologist to collect inner ear tissue from surgical patients that can be used for pathological studies using immunohistochemistry and electron microscopy.
本文介绍了一种手术技术，可在迷路切除术中从黄斑和嵴中获取包含内淋巴管和囊以及前庭上皮的整个前庭导水管。内耳组织用3%戊二醛固定，在0.1 mol/L Na-EDTA中脱钙，并常规处理用于透射电子显微镜检查，包括用四氧化锇后固定。在通过偏高碘酸钠暴露细胞抗原位点后，进行包埋后蛋白A-胶体金免疫电子显微镜检查。为验证该技术，将壶腹嵴和内淋巴囊的薄切片与针对S-100产生的抗体一起孵育，S-100是一种存在于特定类型神经组织中的蛋白质。在壶腹嵴中观察到特异性免疫反应，但在内淋巴囊中未观察到。所描述的手术活检技术为神经耳科医生提供了一种从手术患者中收集内耳组织的方法，该组织可用于使用免疫组织化学和电子显微镜进行病理研究。
