Vukics V, Kery A, Guttman A
Department of Pharmacognosy, Semmelweis University, Budapest, Hungary.
J Chromatogr Sci. 2008 Oct;46(9):823-7. doi: 10.1093/chromsci/46.9.823.
Heartsease (Viola tricolor L.) is a well-known medicinal plant. Its biological activities are supposed to be related to its antioxidant capacity. Garden pansies (Viola x wittrockiana Gams.) have been crossbred from heartsease and are applied as ornamental plants only. In this study, the mother and the daughter species are compared from a phytochemical point of view. Their flavonoid and anthocyanidin contents are determined by spectroscopic methods recommended by the European Pharmacopoeia 5.0. The compositions of the samples (heartsease and garden pansy varietas of several petal color) are analyzed by high-performance liquid chromatography with UV detection and their antioxidant capacity is determined by trolox equivalent antioxidant capacity assay. Our results suggest that garden pansy, especially its flower, is a promising source of natural antioxidants. In addition, a significant correlation is found between the flavonoid content and antioxidant activity.
三色堇（Viola tricolor L.）是一种著名的药用植物。其生物活性被认为与其抗氧化能力有关。园艺三色堇（Viola x wittrockiana Gams.）是由三色堇杂交培育而成，仅用作观赏植物。在本研究中，从植物化学角度对母本和子代物种进行了比较。通过欧洲药典5.0推荐的光谱方法测定它们的黄酮类化合物和花青素含量。采用高效液相色谱-紫外检测法分析样品（几种花瓣颜色的三色堇和园艺三色堇品种）的成分，并通过Trolox当量抗氧化能力测定法测定其抗氧化能力。我们的结果表明，园艺三色堇，尤其是其花朵，是天然抗氧化剂的一个有前景的来源。此外，还发现黄酮类化合物含量与抗氧化活性之间存在显著相关性。
