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三色堇可诱导癌细胞凋亡，并对鸡胚绒毛尿囊膜表现出抗血管生成活性。

Viola tricolor induces apoptosis in cancer cells and exhibits antiangiogenic activity on chicken chorioallantoic membrane.

作者信息

Sadeghnia Hamid Reza, Ghorbani Hesari Taghi, Mortazavian Seyed Mohsen, Mousavi Seyed Hadi, Tayarani-Najaran Zahra, Ghorbani Ahmad

机构信息

Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran ; Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran ; Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran.

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 917751365, Iran.

出版信息

Biomed Res Int. 2014;2014:625792. doi: 10.1155/2014/625792. Epub 2014 Aug 28.

DOI:10.1155/2014/625792
PMID:25243166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4163403/
Abstract

In the present study, the cytotoxic and apoptogenic properties of hydroalcoholic extract and ethyl acetate (EtOAc), n-butanol, and water fractions (0-800 μg/mL) of Viola tricolor were investigated in Neuro2a mouse neuroblastoma and MCF-7 human breast cancer cells. In addition, antiangiogenic effect of EtOAc fraction was evaluated on chicken chorioallantoic membrane (CAM). The quality of EtOAc fraction was also characterized using high performance liquid chromatography (HPLC) fingerprint. Cytotoxicity assay revealed that EtOAc fraction was the most potent among all fractions with maximal effect on MCF-7 and minimal toxicity against normal murine fibroblast L929 cells. Apoptosis induction by EtOAc fraction was confirmed by increased sub-G1 peak of propidium iodide (PI) stained cells. This fraction triggered the apoptotic pathway by increased Bax/Bcl-2 ratio and cleaved caspase-3 level. Moreover, treatment with EtOAc fraction significantly decreased the diameter of vessels on CAM, while the number of newly formed blood vessels was not suppressed significantly. Analysis of quality of EtOAc fraction using HPLC fingerprint showed six major peaks with different retention times. The results of the present study suggest that V. tricolor has potential anticancer property by inducing apoptosis and inhibiting angiogenesis.

摘要

在本研究中，对三色堇水醇提取物及其乙酸乙酯（EtOAc）、正丁醇和水相部分（0 - 800μg/mL）在Neuro2a小鼠神经母细胞瘤细胞和MCF - 7人乳腺癌细胞中的细胞毒性和诱导凋亡特性进行了研究。此外，还评估了EtOAc部分对鸡胚绒毛尿囊膜（CAM）的抗血管生成作用。同时，利用高效液相色谱（HPLC）指纹图谱对EtOAc部分的质量进行了表征。细胞毒性试验表明，EtOAc部分在所有部分中活性最强，对MCF - 7细胞的作用最大，而对正常小鼠成纤维细胞L929的毒性最小。EtOAc部分诱导凋亡得到了碘化丙啶（PI）染色细胞亚G1峰增加的证实。该部分通过提高Bax/Bcl - 2比值和裂解的caspase - 3水平触发凋亡途径。此外，用EtOAc部分处理可显著降低CAM上血管的直径，而对新形成血管的数量没有明显抑制作用。利用HPLC指纹图谱分析EtOAc部分的质量显示出六个具有不同保留时间的主要峰。本研究结果表明，三色堇具有通过诱导凋亡和抑制血管生成发挥潜在抗癌特性的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/05d487e634c0/BMRI2014-625792.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/315113620262/BMRI2014-625792.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/69fd33343801/BMRI2014-625792.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/35b44b84c358/BMRI2014-625792.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/073bff969103/BMRI2014-625792.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/05d487e634c0/BMRI2014-625792.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/315113620262/BMRI2014-625792.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/69fd33343801/BMRI2014-625792.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/35b44b84c358/BMRI2014-625792.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/073bff969103/BMRI2014-625792.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9794/4163403/05d487e634c0/BMRI2014-625792.007.jpg

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