Kaneko Yui, Lindsay Laura A, Murphy Christopher R
School of Medical Sciences, The Bosch Institute, The University of Sydney, NSW, Australia.
Reprod Fertil Dev. 2008;20(8):892-9. doi: 10.1071/rd08148.
During early pregnancy in rodents, invasion of the blastocyst into the endometrial decidual cells is accompanied by the removal of uterine epithelial cells around the implantation sites. The present study investigated the distribution and expression of two focal adhesion proteins, namely talin and paxillin, in rat uterine epithelial cells during early pregnancy and their role in the loss of these cells at the time of implantation. A major distributional change of talin and paxillin was demonstrated in uterine epithelial cells during early pregnancy. From a highly concentrated expression along the basal cell surface on Day 1 of pregnancy, talin and paxillin were lost from the basal cell surface at the time of implantation. There was also a corresponding statistically significant decrease in paxillin seen through western blotting analysis. Together, these observations suggest that uterine epithelial cells are less adherent to the underlying basal lamina due to the disassembly of talin and paxillin from focal adhesions, facilitating removal of these cells at the time of implantation. This phenomenon was restricted to the period of receptivity because talin and paxillin reappeared along the basal cell surface soon after implantation.
在啮齿动物的妊娠早期，胚泡侵入子宫内膜蜕膜细胞的过程伴随着植入部位周围子宫上皮细胞的移除。本研究调查了两种粘着斑蛋白（即踝蛋白和桩蛋白）在大鼠妊娠早期子宫上皮细胞中的分布和表达情况，以及它们在植入时这些细胞丢失过程中所起的作用。研究表明，妊娠早期子宫上皮细胞中踝蛋白和桩蛋白的分布发生了主要变化。从妊娠第1天沿着基底细胞表面高度集中表达开始，植入时踝蛋白和桩蛋白从基底细胞表面消失。通过蛋白质免疫印迹分析还发现桩蛋白相应地有统计学意义的减少。这些观察结果共同表明，由于踝蛋白和桩蛋白从粘着斑上解离，子宫上皮细胞与下方基底膜的粘附性降低，这有利于在植入时移除这些细胞。这种现象仅限于接受期，因为植入后不久，踝蛋白和桩蛋白又重新出现在基底细胞表面。
