Kaneko Yui, Lecce Laura, Day Margot L, Murphy Christopher R
School of Medical Sciences (Discipline of Anatomy and Histology) and The Bosch Institute, The University of Sydney, Sydney, NSW 2006, Australia.
J Morphol. 2012 Jun;273(6):639-50. doi: 10.1002/jmor.20010. Epub 2012 Feb 10.
Focal adhesions play an important role in promoting embryo invasion; in particular, focal adhesions disassemble at the time of implantation in the rat, facilitating the detachment of the uterine luminal epithelium to allow the embryo to invade the endometrium. This study investigated focal adhesion protein, focal adhesion kinase (FAK) in the rat uterine luminal, and glandular epithelial cells to understand the dynamics of focal adhesions during early pregnancy. FAK undergoes extensive distributional change during early pregnancy, and surprisingly, FAK was not localized at the site of focal adhesions, instead being localized to the site of cell-to-cell contact and colocalizing with ZO-1 on day 1 of pregnancy. At the time of implantation, FAK increases in the apical region of the uterine luminal epithelial cells which was regulated by progesterone. Using an in vitro co-culture model of rat blastocysts attached to Ishikawa cells, FAK was present apically both in the rat blastocyst and the Ishikawa cells, suggesting a role in attachment andin mediating signal transduction between these two genetically different cell types.
粘着斑在促进胚胎侵入方面发挥着重要作用；特别是，在大鼠着床时粘着斑会解体，促进子宫腔上皮的分离，使胚胎能够侵入子宫内膜。本研究调查了大鼠子宫腔和腺上皮细胞中的粘着斑蛋白——粘着斑激酶（FAK），以了解妊娠早期粘着斑的动态变化。FAK在妊娠早期经历广泛的分布变化，令人惊讶的是，FAK并不定位于粘着斑部位，而是定位于细胞间接触部位，并在妊娠第1天与紧密连接蛋白1（ZO-1）共定位。在着床时，子宫腔上皮细胞顶端区域的FAK增加，这是由孕酮调节的。使用大鼠囊胚与石川细胞附着的体外共培养模型，FAK在大鼠囊胚和石川细胞的顶端均有存在，表明其在这两种基因不同的细胞类型之间的附着和介导信号转导中发挥作用。
