Nicholson Leigh, Madawala Romanthi, Lindsay Laura, Murphy Christopher R
Cell and Reproductive Biology Lab, Discipline of Anatomy and Histology, School of Medical Sciences, University of Sydney, Camperdown, Australia.
Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, Australia.
Histochem Cell Biol. 2019 May;151(5):395-406. doi: 10.1007/s00418-018-1761-y. Epub 2018 Dec 5.
During early pregnancy, the uterine luminal epithelial cells (UECs) and endometrial stromal cells (ESCs) undergo morphological changes to enable blastocyst implantation. The present study investigates, for the first time, the cytoskeletal-associated proteins and α-actinin superfamily members, α-parvin and β-parvin, during early pregnancy in the rat uterus. These two PARVA proteins are involved in cell adhesion, morphological changes and regulation of other cytoskeletal proteins, through binding with proteins such as actin and integrin-linked kinase. α-parvin is present in UECs at fertilisation and significantly decreases by the time of implantation. β-parvin acts in opposition; significantly increasing in both UECs and ESCs at the time of implantation, suggesting a role in the process of decidualisation. Additionally, the presence of a serine-8 residue-phosphorylated α-parvin, which is associated with cell morphology changes, was found in the nuclear region of both UECs and ESCs during implantation and decidualisation. We also show that the presence of both β-parvin and phosphorylated α-parvin in ESCs is dependent on decidualisation occurring. This study demonstrates that the changing balance and localisation of the two PARVA proteins are dependent on the time of uterine receptivity, suggesting a co-dependent role in the cytoskeletal re-organisation crucial to the changing conditions necessary for implantation and decidualisation.
在妊娠早期，子宫腔上皮细胞（UECs）和子宫内膜基质细胞（ESCs）会发生形态变化，以促进囊胚着床。本研究首次调查了大鼠子宫妊娠早期细胞骨架相关蛋白以及α-辅肌动蛋白超家族成员α-帕文和β-帕文。这两种PARVA蛋白通过与肌动蛋白和整合素连接激酶等蛋白质结合，参与细胞黏附、形态变化以及其他细胞骨架蛋白的调节。α-帕文在受精时存在于UECs中，到着床时显著减少。β-帕文则相反，在着床时UECs和ESCs中均显著增加，表明其在蜕膜化过程中发挥作用。此外，在着床和蜕膜化过程中，在UECs和ESCs的核区域均发现了与细胞形态变化相关的丝氨酸-8残基磷酸化α-帕文。我们还表明，ESCs中β-帕文和磷酸化α-帕文的存在取决于蜕膜化的发生。本研究表明，两种PARVA蛋白的平衡和定位变化取决于子宫接受性的时间，提示它们在对着床和蜕膜化所需变化条件至关重要的细胞骨架重组中具有共同依赖作用。
