用于基因克隆和翻译融合的乳球菌载体家族的构建。
Construction of a family of lactococcal vectors for gene cloning and translational fusion.
作者信息
Xu F F, Pearce L E, Yu P L
机构信息
Department of Biotechnology, Massey University, Palmerston North, New Zealand.
出版信息
FEMS Microbiol Lett. 1991 Jan 1;61(1):55-9. doi: 10.1111/j.1574-6968.1991.tb04321.x.
Abstract
A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion studies in lactococci.
摘要
基于pFX1复制子构建了一系列稳定的乳球菌载体，这些载体使用α片段或完整的大肠杆菌lacZ基因作为选择标记。这些载体还包含多个克隆位点，并给出了它们在乳球菌中用于基因克隆和翻译融合研究的示例。
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