Rexroad C E, Powell A M
Livest. and Poult. Sci. Inst. ARS, Beltsville, MD 20705.
J Anim Sci. 1991 Jan;69(1):246-51. doi: 10.2527/1991.691246x.
In Exp. 1, five injections of crude FSH (20 mg total), starting 36 h before removal of progestin pessaries or injection of prostaglandin F2 alpha (PGF2 alpha), were found to be as effective for superovulation of ewes as seven injections starting 72 h before removal of pessaries. Seven injections caused superovulation (more than three ovulations) in 13 of 15 ewes with an average (all ewes) of 12 +/- 2.3 ovulations. Five injections caused superovulation in 17 of 19 ewes with an average of 11.5 +/- 1.6 ovulations after pessary-regulated estrus in 12 of 16 ewes with an average of 12.4 +/- 2.2 ovulations after PGF2 alpha injection. In Exp. 2, intrauterine artificial insemination (IUAI) of naturally mated ewes provided ovum fertilization rates of 75.3% with an embryo recovery rate of 39.4%, whereas mating alone (MATED) provided fertilization rates of 64.2% with an embryo recovery rate of 72.1%. However, subsequent replications of the MATED protocol (MATED2) resulted in a low fertilization rate (34.2%). In Exp. 3, surgical IUAI at 22 h after removal of pessaries resulted in a low rate of superovulation (5 of 12 ewes), presumably as the result of pentobarbital anesthesia. Conducting IUAI at 46 h after removal of pessaries resulted in a high rate of superovulation (14 of 16 ewes) but a low embryo recovery rate (30.7%). In two subsequent trials, IUAI at 40 h after removal of pessaries resulted in most ewes superovulating (33 of 35 in April/May and 16 of 17 in September) with acceptable embryo recovery (65.2% and 71.3%) and high fertilization rates (82.8% and 96.4%).(ABSTRACT TRUNCATED AT 250 WORDS)
在实验1中，在取出孕酮阴道栓或注射前列腺素F2α（PGF2α）前36小时开始，注射5次粗制促卵泡素（总量20毫克），对母羊超数排卵的效果与在取出阴道栓前72小时开始注射7次的效果相同。7次注射使15只母羊中的13只出现超数排卵（排卵超过3个），所有母羊平均排卵数为12±2.3个。5次注射使19只母羊中的17只出现超数排卵，在16只经阴道栓调节发情的母羊中，平均排卵数为12.4±2.2个，在12只注射PGF2α后的母羊中，平均排卵数为11.5±1.6个。在实验2中，对自然交配的母羊进行子宫内人工授精（IUAI），卵子受精率为75.3%，胚胎回收率为39.4%，而仅进行交配（MATED）的受精率为64.2%，胚胎回收率为72.1%。然而，随后重复MATED方案（MATED2）导致受精率较低（34.2%）。在实验3中，在取出阴道栓后22小时进行手术子宫内人工授精，超数排卵率较低（12只母羊中有5只），推测是戊巴比妥麻醉的结果。在取出阴道栓后46小时进行子宫内人工授精，超数排卵率较高（16只母羊中有14只），但胚胎回收率较低（30.7%）。在随后的两项试验中，在取出阴道栓后40小时进行子宫内人工授精，大多数母羊出现超数排卵（4月/5月的35只中有33只，9月的17只中有16只），胚胎回收率可接受（分别为65.2%和71.3%），受精率较高（分别为82.8%和96.4%）。（摘要截短至250字）
