Wright R W, Bondioli K, Grammer J, Kuzan F, Menino A
J Anim Sci. 1981 Jan;52(1):115-8. doi: 10.2527/jas1981.521115x.
The estrous cycles of 28 ewes 9 to 10 months of age were synchronized with Medoxyprogesterone (MAP) pessaries. Superovulation was attempted by injecting either (follicle stimulating hormone) FSH or FSH plus Luteinizing Hormone (LH). MAP pessaries were in place for 12 days. FSH was administered (IM) to all ewes at 12-hr intervals over a 3-day period, 5 mg injected twice on day 11 after pessary insertion, followed by 4 and 3 mg twice daily on days 12 and 13, respectively. LH (25 mg injected IV) was given to 14 ewes within 8 hr after the onset of behavioral estrus. All ewes were hand mated with several rams at 12-hr intervals from the onset to the end of behavioral estrus. Ovulation, embryo recovery and fertilization rates were recorded for each ewe. Of 14 ewes injected with only FSH, 13 ovulated, with a mean ovulation rate of 8.2 +/- 5.6; embryo recovery rate averaged 62%, and fertilization rate was 97%. All 14 ewes given both FSH + LH ovulated, with an ovulation rate of 8.9 +/- 5.8 per ewe; embryo recovery averaged 70%, and 91% of all embryos recovered were fertilized. No statistical differences in ovulation, embryo recovery or fertilization rates were found between the FSH and FSH + LH superovulation treatment groups. In addition, no difference in the number of ewes showing estrus or duration of behavioral estrus was noted between FSH and FSH + LH treatments.
选用甲孕酮（MAP）阴道栓使28只9至10月龄母羊的发情周期同步。通过注射促卵泡素（FSH）或FSH加促黄体素（LH）尝试进行超数排卵。MAP阴道栓放置12天。在3天内每隔12小时对所有母羊进行一次促卵泡素（FSH）肌肉注射，在阴道栓插入后第11天注射5毫克，分两次注射，随后在第12天和第13天分别每天两次注射4毫克和3毫克。14只母羊在出现行为性发情后8小时内静脉注射促黄体素（LH，25毫克）。从行为性发情开始到结束，每隔12小时用几只公羊对所有母羊进行人工授精。记录每只母羊的排卵、胚胎回收和受精率。在仅注射FSH的14只母羊中，13只排卵，平均排卵率为8.2±5.6；胚胎回收率平均为62%，受精率为97%。接受FSH + LH的14只母羊全部排卵，每只母羊的排卵率为8.9±5.8；胚胎回收率平均为70%，回收的所有胚胎中有91%受精。在FSH和FSH + LH超数排卵治疗组之间，排卵、胚胎回收或受精率没有统计学差异。此外，在FSH和FSH + LH治疗组之间，出现发情的母羊数量或行为性发情持续时间没有差异。
