Hoerder-Suabedissen Anna, Wang Wei Zhi, Lee Sheena, Davies Kay E, Goffinet André M, Rakić Sonja, Parnavelas John, Reim Kerstin, Nicolić Margareta, Paulsen Ole, Molnár Zoltán
Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.
Cereb Cortex. 2009 Aug;19(8):1738-50. doi: 10.1093/cercor/bhn195. Epub 2008 Nov 13.
The subplate lays the foundation of the developing cerebral cortex, and abnormalities have been suggested to contribute to various brain developmental disorders. The causal relationship between cellular pathologies and cognitive disorders remains unclear, and therefore, a better understanding of the role of subplate cells in cortical development is essential. Only by determining the molecular taxonomy of this diverse class of neurons can we identify the subpopulations that may contribute differentially to cortical development. We identified novel markers for murine subplate cells by comparing gene expression of subplate and layer 6 of primary visual and somatosensory cortical areas of postnatal day (P)8 old mice using a microarray-based approach. We examined the utility of these markers in well-characterized mutants (reeler, scrambler, and p35-KO) where the subplate is displaced in relation to the cortical plate. In situ hybridization or immunohistochemistry confirmed subplate-selective expression of complexin 3, connective tissue growth factor, nuclear receptor-related 1/Nr4a2, and monooxygenase Dbh-like 1 while transmembrane protein 163 also had additional expression in layer 5, and DOPA decarboxylase was also present in the white matter. Localization of marker-positive cells in the reeler and p35-KO cortices suggests different subpopulations of subplate cells. These new markers open up possibilities for further identification of subplate subpopulations in research and in neuropathological diagnosis.
皮层下板为发育中的大脑皮层奠定了基础，并且有研究表明其异常与多种脑发育障碍有关。细胞病理学与认知障碍之间的因果关系仍不明确，因此，更好地了解皮层下板细胞在皮层发育中的作用至关重要。只有确定这类多样神经元的分子分类，我们才能识别出可能对皮层发育有不同贡献的亚群。我们通过基于微阵列的方法，比较出生后第8天（P8）小鼠初级视觉和体感皮层区域的皮层下板和第6层的基因表达，确定了小鼠皮层下板细胞的新标记物。我们在特征明确的突变体（reeler、scrambler和p35-KO）中检测了这些标记物的效用，在这些突变体中，皮层下板相对于皮质板发生了移位。原位杂交或免疫组织化学证实了复合体3、结缔组织生长因子、核受体相关蛋白1/Nr4a2和单加氧酶Dbh样蛋白1在皮层下板的选择性表达，而跨膜蛋白163在第5层也有额外表达，多巴脱羧酶在白质中也有表达。标记阳性细胞在reeler和p35-KO皮层中的定位表明皮层下板细胞存在不同亚群。这些新标记物为在研究和神经病理学诊断中进一步识别皮层下板亚群开辟了可能性。
