Yilmaz Y, Guler C
Department of Pedodontics, Ataturk University, Faculty of Dentistry, Erzurum, Turkey.
J Indian Soc Pedod Prev Dent. 2008 Dec;26(4):162-7. doi: 10.4103/0970-4388.44032.
The aim of this study was to evaluate the changes caused by different sterilization or disinfection methods on the vestibular surface of four commercially made preformed crowns using stereomicroscopy and scanning electron microscopy (SEM). Preformed crowns (NuSmile Primary Anterior Crown (NSC), Kinder Krowns (KK), Pedo Pearls (PP) and polycarbonate crowns (PC)) were sterilized and/or disinfected by one of the following techniques: no sterilization or disinfection (G1 control group); steam autoclaving at 134 degrees C (30 psi) for 4 min (G2); steam autoclaving at 134 degrees C (30 psi) for 12 min (G3); steam autoclaving at 121 degrees C (15 psi) for 30 min (G4); and ultrasonication in a bath containing 4% Lysetol AF for 5 min at room temperature (chemical disinfection) (G5). Scanning electron micrographs of the crowns were taken before and after their sterilization or disinfection. The changes on the vestibular surface were then scored for the presence or absence of crazing, contour alteration, fracturing, and vestibular surface changes. The data were analyzed statistically using the chi-square test. No changes were observed before and after sterilization or disinfection in the stereomicroscopic evaluation of the vestibular surface of the crowns. However, all methods in which steam autoclaving was used to sterilize the crowns caused significant (P < 0.05) crazing and contour alterations of the vestibular surface of the crowns when they were examined by SEM. Chemical disinfection using an aldehyde-free disinfectant is the preferred method of disinfection for crowns that have been used previously in other dental patients.
本研究的目的是使用体视显微镜和扫描电子显微镜(SEM)评估不同灭菌或消毒方法对四种市售预成冠的前庭表面所造成的变化。预成冠(NuSmile乳牙前牙冠(NSC)、儿童冠(KK)、儿童珍珠冠(PP)和聚碳酸酯冠(PC))通过以下技术之一进行灭菌和/或消毒:不进行灭菌或消毒(G1对照组);在134摄氏度(30磅力/平方英寸)下蒸汽高压灭菌4分钟(G2);在134摄氏度(30磅力/平方英寸)下蒸汽高压灭菌12分钟(G3);在121摄氏度(15磅力/平方英寸)下蒸汽高压灭菌30分钟(G4);以及在室温下于含有4% Lysetol AF的浴中超声处理5分钟(化学消毒)(G5)。在冠进行灭菌或消毒前后拍摄其扫描电子显微照片。然后对前庭表面的变化进行评分,以确定是否存在裂纹、外形改变、破裂和前庭表面变化。使用卡方检验对数据进行统计学分析。在对冠的前庭表面进行体视显微镜评估时,灭菌或消毒前后未观察到变化。然而,当通过SEM检查时,所有使用蒸汽高压灭菌冠的方法均导致冠的前庭表面出现显著(P < 0.05)的裂纹和外形改变。对于先前在其他牙科患者中使用过的冠,使用无醛消毒剂进行化学消毒是首选的消毒方法。