Wang Zhao, Cai Shi-Rong, He Yu-Long, Zhan Wen-Hua, Chen Chuang-Qi, Cui Ji, Wu Wen-Hui, Wu Hui, Song Wu, Zhang Chang-Hua, Peng Jian-Jun, Huang Xiao-Hui
Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China.
Ann Surg Oncol. 2009 Jan;16(1):208-19. doi: 10.1245/s10434-008-0214-6. Epub 2008 Nov 14.
High expression of PRL-3 had been implicated in lymph node metastasis of gastric cancer. In the present study, we detected the expression of PRL-3 in primary gastric cancer tissue, and evaluated its role in gastric cancer growth and the prognostic impact on patients. PRL-3 phosphatase expression was measured in 137 gastric tumor samples by using the immunohistochemistry method, and the overall survival rate was compared between the patients with high PRL-3 expression (n = 85) and those with moderate or low PRL-3 expression (n = 52). RNA interference, mediated by recombinant lentivirus expressing artificial PRL-3 miRNA, was used to knockdown PRL-3 expression in SGC7901 cell line. MTT assay and animal experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and tumor growth. PRL-3 expression was more frequently detected in tumors with a diameter >40 mm and in advanced stages. Furthermore, the overall survival rate of high PRL-3 expression was significantly lower than that of moderate or low PRL-3 expression (P < 0.001), and multivariate analysis showed that PRL-3 expression level independently influences the survival of patients (P = 0.024). Importantly, knockdown of PRL-3 significantly suppressed the proliferation of SGC7901 cells and slowed the tumor growth compared with controls (P < 0.05). PRL-3 is associated with gastric cancer progression. High PRL-3 expression in the primary lesion had a negative impact on prognosis. PRL-3 plays a key role in the control of gastric cancer growth. PRL-3 should be considered as a potential therapeutic target and a prognostic factor.
PRL-3的高表达与胃癌的淋巴结转移有关。在本研究中，我们检测了原发性胃癌组织中PRL-3的表达，并评估了其在胃癌生长中的作用以及对患者预后的影响。采用免疫组织化学方法检测了137例胃肿瘤样本中PRL-3磷酸酶的表达，并比较了PRL-3高表达患者（n = 85）和PRL-3中低表达患者（n = 52）的总生存率。利用表达人工PRL-3 miRNA的重组慢病毒介导的RNA干扰技术，敲低SGC7901细胞系中PRL-3的表达。通过MTT法和动物实验确定PRL-3在SGC7901细胞增殖和肿瘤生长中的作用。在直径>40 mm的肿瘤和晚期肿瘤中更频繁地检测到PRL-3表达。此外，PRL-3高表达患者的总生存率显著低于PRL-3中低表达患者（P < 0.001），多因素分析显示PRL-3表达水平独立影响患者的生存（P = 0.024）。重要的是，与对照组相比，敲低PRL-3可显著抑制SGC7901细胞的增殖并减缓肿瘤生长（P < 0.05）。PRL-3与胃癌进展相关。原发灶中PRL-3的高表达对预后有负面影响。PRL-3在控制胃癌生长中起关键作用。PRL-3应被视为潜在的治疗靶点和预后因素。
