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肝再生磷酸酶-3 诱导的 miR-21、miR-17 和 miR-19a 促进结肠癌的增殖和转移。

miR-21, miR-17 and miR-19a induced by phosphatase of regenerating liver-3 promote the proliferation and metastasis of colon cancer.

机构信息

Department of Hepatobiliary Surgery, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, 107 Yanjiang West Road, Guangzhou 510120, China.

出版信息

Br J Cancer. 2012 Jul 10;107(2):352-9. doi: 10.1038/bjc.2012.251. Epub 2012 Jun 7.

DOI:10.1038/bjc.2012.251
PMID:22677902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3394980/
Abstract

BACKGROUND

Phosphatase of regenerating liver-3 (PRL-3) is an oncogene known to promote tumour metastasis, especially in colorectal cancer (CRC). Here, we demonstrate that the miR-21, miR-17 and miR-19a expressions induced by PRL-3 are involved in the proliferation and metastasis of colon cancer.

METHODS

Microarray analysis and quantitative reverse-transcription polymerase chain reactions (qRT-PCR) were used to investigate the changes in miRNA expression due to the overexpression of PRL-3. Transwell chamber invasion assays, CCK-8 proliferation assays and RNA interference assays were used to explore the effects of PRL-3 on miR-21, miR-17 and miR-19a expression in colon cancer cells. Immunohistochemistry and qRT-PCR were performed in colon cancer tissues to evaluate the expression of PRL-3, signal transducer and activator of transcription 3 (STAT3), miR-21, miR-17 and miR-19a.

RESULTS

Our study demonstrated that the overexpression of PRL-3 in colon cancer cells induced the expression of miR-21, miR-17 and miR-19a by activating STAT3. Subsequently, these microRNAs contributed to the increased proliferation and invasiveness of the colon cancer cells. Positive correlations between PRL-3 and these microRNAs were also observed in matched primary colon cancer tissues and metastatic lesions.

CONCLUSION

miR-21, miR-17 and miR-19a induced by PRL-3 contribute to the proliferation and invasion of colon cancer.

摘要

背景

肝再生磷酸酶-3（PRL-3）是一种已知可促进肿瘤转移的癌基因，尤其是在结直肠癌（CRC）中。在这里，我们证明了 PRL-3 诱导的 miR-21、miR-17 和 miR-19a 的表达参与了结肠癌的增殖和转移。

方法

使用微阵列分析和定量逆转录聚合酶链反应（qRT-PCR）来研究由于 PRL-3 的过表达而导致的 miRNA 表达变化。Transwell 室侵袭实验、CCK-8 增殖实验和 RNA 干扰实验用于探索 PRL-3 对结肠癌细胞中 miR-21、miR-17 和 miR-19a 表达的影响。免疫组织化学和 qRT-PCR 用于评估结肠癌组织中 PRL-3、信号转导和转录激活因子 3（STAT3）、miR-21、miR-17 和 miR-19a 的表达。

结果

我们的研究表明，PRL-3 在结肠癌细胞中的过表达通过激活 STAT3 诱导 miR-21、miR-17 和 miR-19a 的表达。随后，这些 microRNAs 促进了结肠癌细胞的增殖和侵袭。在匹配的原发性结肠癌组织和转移性病变中，也观察到了 PRL-3 与这些 microRNAs 之间的正相关。

结论

PRL-3 诱导的 miR-21、miR-17 和 miR-19a 促进了结肠癌的增殖和侵袭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/e53e9e4fa98a/bjc2012251f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/384b4d853020/bjc2012251f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/55f8212e43dc/bjc2012251f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/bf74c3454c46/bjc2012251f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/72fba61539d8/bjc2012251f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/5e91eb938bcd/bjc2012251f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/e53e9e4fa98a/bjc2012251f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/384b4d853020/bjc2012251f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/55f8212e43dc/bjc2012251f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/bf74c3454c46/bjc2012251f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/72fba61539d8/bjc2012251f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/5e91eb938bcd/bjc2012251f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ca9/3394980/e53e9e4fa98a/bjc2012251f6.jpg

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本文引用的文献

1
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Oncol Rep. 2011 Oct;26(4):909-17. doi: 10.3892/or.2011.1366. Epub 2011 Jun 28.
2
Caveolin-1 upregulation mediates suppression of primary breast tumor growth and brain metastases by stat3 inhibition.窖蛋白-1 的上调介导了通过 stat3 抑制对原发性乳腺癌生长和脑转移的抑制。
Cancer Res. 2011 Jul 15;71(14):4932-43. doi: 10.1158/0008-5472.CAN-10-4249. Epub 2011 May 27.
3
Stat3 and MMP7 contribute to pancreatic ductal adenocarcinoma initiation and progression.
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4
Protein Tyrosine Phosphatase PRL-3: A Key Player in Cancer Signaling.蛋白酪氨酸磷酸酶 PRL-3：癌症信号转导中的关键分子。
Biomolecules. 2024 Mar 12;14(3):342. doi: 10.3390/biom14030342.
5
Screening of miRNAs as prognostic biomarkers and their associated hub targets across Hepatocellular carcinoma using survival-based bioinformatics approach.基于生存的生物信息学方法筛选肝细胞癌中作为预后生物标志物的miRNA及其相关枢纽靶点。
J Genet Eng Biotechnol. 2024 Mar;22(1):100337. doi: 10.1016/j.jgeb.2023.100337. Epub 2024 Jan 24.
6
microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms.microRNAs：通过内源性和跨物种机制调节植物对非生物和生物胁迫响应的关键调控因子。
Int J Mol Sci. 2024 Jan 18;25(2):1154. doi: 10.3390/ijms25021154.
7
PRL3 as a therapeutic target for novel cancer immunotherapy in multiple cancer types.PRL3 作为多种癌症新型癌症免疫疗法的治疗靶点。
Theranostics. 2023 Mar 21;13(6):1876-1891. doi: 10.7150/thno.79265. eCollection 2023.
8
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10
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4
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5
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Otol Neurotol. 2010 Dec;31(9):1455-62. doi: 10.1097/MAO.0b013e3181f20655.
6
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7
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9
MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC).微小 RNA-21（miR-21）抑制肿瘤抑制因子 PTEN，促进非小细胞肺癌（NSCLC）的生长和侵袭。
Clin Chim Acta. 2010 Jun 3;411(11-12):846-52. doi: 10.1016/j.cca.2010.02.074. Epub 2010 Mar 16.
10
Constitutively active Stat3 enhances neu-mediated migration and metastasis in mammary tumors via upregulation of Cten.组成性激活的 Stat3 通过上调 Cten 增强了乳腺肿瘤中的神经介导的迁移和转移。
Cancer Res. 2010 Mar 15;70(6):2558-67. doi: 10.1158/0008-5472.CAN-09-2840. Epub 2010 Mar 9.