一种用于丙型肝炎病毒基因分型的实时Taqman方法及分离株进一步亚型分型的方法。
A real-time Taqman method for hepatitis C virus genotyping and methods for further subtyping of isolates.
作者信息
Rolfe Kathryn J, Wreghitt Tim G, Alexander Graeme J M, Curran Martin D
机构信息
Health Protection Agency Cambridge Microbiology and Public Health Laboratory Addenbrooke's Hospital, Cambridge, UK.
出版信息
Methods Mol Biol. 2009;510:55-71. doi: 10.1007/978-1-59745-394-3_5.
The HCV genome is highly heterogeneous; more and more genotypes, each with several distinct subtypes, are being identified around the world. Knowledge of genotype is important for planning of treatment regimes, whereas subtype identification is useful in epidemiological studies and outbreak investigation. We describe HCV genotyping and subtyping assays, based on real-time PCR, that are sensitive, specific, and reliable. These assays provide fast, accurate, and convenient methods for HCV genotyping/subtyping to support clinical practice.
丙型肝炎病毒（HCV）基因组具有高度异质性；世界各地正在识别出越来越多的基因型，每种基因型又有几个不同的亚型。了解基因型对于治疗方案的规划很重要，而亚型鉴定在流行病学研究和疫情调查中很有用。我们描述了基于实时聚合酶链反应（PCR）的HCV基因分型和亚型分型检测方法，这些方法灵敏、特异且可靠。这些检测方法为HCV基因分型/亚型分型提供了快速、准确且便捷的方法，以支持临床实践。
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