1型糖尿病伴或不伴糖尿病肾病患者循环血液中血小板-淋巴细胞聚集情况

Status of platelet-lymphocyte aggregation in circulating blood of patients with type 1 diabetes with and without diabetic nephropathy.

作者信息

Vitkovsky Yuri, Kuznik Boris, Solpov Alexey, Magen Eli

机构信息

Department of Physiology, Chita Medical Academy, Chita, Russia.

出版信息

Isr Med Assoc J. 2008 Oct;10(10):691-4.

PMID:19009947

Abstract

BACKGROUND

Platelets can modulate the role of lymphocytes in the development of micro- and macrovascular complications in type 1 diabetes mellitus.

OBJECTIVES

To clarify the status of platelet-lymphocyte aggregation in circulating blood in patients with T1DM, as well as the differences in the platelet-lymphocyte aggregation in T1DM patients with and without diabetic nephropathy.

METHODS

We recruited 115 T1DM patients (47 men and 68 women) aged 15-52 years. The subjects with mean albumin excretion > or = 5 microg/mg creatinine comprised group 1, and those with < 5 microg/mg creatinine comprised group 2. The matched healthy participants (n=50) served as the control group. Detection of LPA was achieved using a light microscope after Ficoll-gradient centrifugation. Immunophenotyping of lymphocytes was performed by flow cytometry.

RESULTS

Significantly more LPA (430.4 +/- 20.6/microl) were observed in group 2 compared with group 1 (223.9 +/- 12.8/microl, P< 0.001) and the control group (296.1 +/- 22.6/microl, P=0.027). In group 1 significantly more LPA/CD4 (21.1 +/- 1.6%) and LPA/(CD4 + NK) (17.8 +/- 1.7%) were found than in group 2 and the control group.

CONCLUSION

T1DM with diabetic nephropathy is associated with higher levels of LPA than T1MD without diabetic nephropathy. The role of LPA in microvascular complications in diabetes should be elucidated in further studies.

摘要

背景

血小板可调节1型糖尿病微血管和大血管并发症发生过程中淋巴细胞的作用。

目的

阐明1型糖尿病患者循环血液中血小板 - 淋巴细胞聚集的状态，以及有无糖尿病肾病的1型糖尿病患者血小板 - 淋巴细胞聚集的差异。

方法

我们招募了115例年龄在15 - 52岁的1型糖尿病患者（47例男性和68例女性）。平均白蛋白排泄量≥5微克/毫克肌酐的受试者为第1组，<5微克/毫克肌酐的受试者为第2组。匹配的健康参与者（n = 50）作为对照组。采用Ficoll梯度离心后用光学显微镜检测淋巴细胞血小板聚集（LPA）。通过流式细胞术进行淋巴细胞免疫表型分析。

结果

与第1组（223.9±12.8/微升，P < 0.001）和对照组（296.1±22.6/微升，P = 0.027）相比，第2组观察到的LPA显著更多（430.4±20.6/微升）。与第2组和对照组相比，第1组中发现的LPA/CD4（21.1±1.6%）和LPA/(CD4 + NK)（17.8±1.7%）显著更多。