Forest M G
Horm Res. 1976;7(4-5):260-73. doi: 10.1159/000178737.
A highly specific antiserum raised against the 3(O-carboxymethyl)oxime of 17alpha-hydroxyprogesterone (17-OHP) was produced in rabbit and used in the development of a specific radioimmunoassay (RIA) for 17-OHP which included a celite chromatography. Methods allowing the measurement of plasma 17-OHP levels either separately or in combination with that of several plasma androgens (testosterone, delta4-androstenedione and dehydroepiandrosterone) are described. Moreover, RIA meabsurement of plasma 17-OHP levels with or without chromatographic (celite column) purification gave comparable results (mean +/- SD) in 29 normal adult males (118 +/- 34 ng/100 ml) and 35 normal adult females (follicular phase: 46+/- 16 ng/100 ml); luteal phase: 241 +/- 71 ng/100 ml).
用17α-羟孕酮（17-OHP）的3（O-羧甲基）肟免疫家兔制备了一种高特异性抗血清，并将其用于开发一种针对17-OHP的特异性放射免疫分析（RIA），该分析包括硅藻土色谱法。本文描述了分别或与几种血浆雄激素（睾酮、Δ4-雄烯二酮和脱氢表雄酮）联合测量血浆17-OHP水平的方法。此外，在29名正常成年男性（118±34 ng/100 ml）和35名正常成年女性（卵泡期：46±16 ng/100 ml；黄体期：241±71 ng/100 ml）中，经色谱（硅藻土柱）纯化或未经纯化的血浆17-OHP水平的RIA测量结果相当（均值±标准差）。
