Hami M, Rosler A, Rabinowitz D
J Clin Endocrinol Metab. 1975 May;40(5):863-7. doi: 10.1210/jcem-40-5-863.
A radioimmunoassay is described for the measurement of 17alpha-hydroxyprogesterone in human plasma. We have employed the principle of "immunologic purification." 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether. The 17-OHP is subsequently freed from antibody by acid hydrolysis and this extract is assayed by radioimmunoassay.
本文描述了一种用于测定人血浆中17α-羟孕酮的放射免疫分析法。我们采用了“免疫纯化”原理。17-OHP及相关类固醇与过量抗血清结合。对抗体亲和力低的类固醇用乙醚萃取。随后通过酸水解使17-OHP从抗体上解离，然后用放射免疫分析法对该提取物进行检测。
