Nishihara M, Koga Y
Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu, Japan.
Biochim Biophys Acta. 1991 Mar 12;1082(2):211-7. doi: 10.1016/0005-2760(91)90196-o.
Lipids of the methanogenic archaebacterium, Methanosarcina barkeri were analyzed. The lipid content was 5.4% of dry cell and polar lipids comprised 87% of the total lipid. Polar lipids were separated into 14 spots by two-dimensional thin-layer chromatography. These were six phospholipids, seven aminophospholipids and one glycolipid, of which two phospholipids and two aminophospholipids were major constituents. After removal of polar head groups from total lipids, two kinds of glycerol diether core lipids were found. One was 2,3-di-O-phytanyl-sn-glycerol (archaeol) and the other 2-O-(3'-hydroxy-3', 7', 11', 15'-tetramethyl)hexadecyl-3-O-phytanyl-sn- glycerol (hydroxyarchaeol). Those structures were identified on the basis of chemical analysis, fast atom bombardment spectrometry, gas-liquid chromatography-mass spectrometry and 1H- and 13C-NMR spectrometry. The latter was a new core lipid which was different from hydroxyarchaeol of Methanothrix concilii. The hydroxyarchaeol core lipid comprised 60% of polar lipid in M. barkeri. The structures of core lipids are quite different from those previously reported by De Rosa et al. (Biochim, Biophys. Acta (1986) 875, 487-492) concerning M. barkeri lipids. The structures of two major polar lipids, both of which had hydroxyarchaeol as core proteins, were elucidated. These lipids were 2-O-(3'-hydroxy)phytanyl-3-O-phytanyl-sn-glycero-1-phosphoserine (hydroxyarchaetidylserine) and 2-O-(3'-hydroxy)phytanyl-3-O-phytanyl-sn- glycerol-phospho-myo-inositol (hydroxyarchaetidyl-myo-inositol). Archaetidylserine and archaetidylinositol, which had the usual archaeol core portion, were also present as minor polar lipids.
对产甲烷古细菌巴氏甲烷八叠球菌的脂质进行了分析。脂质含量占干细胞的5.4%，极性脂质占总脂质的87%。通过二维薄层色谱法将极性脂质分离为14个斑点。其中有六种磷脂、七种氨基磷脂和一种糖脂，其中两种磷脂和两种氨基磷脂是主要成分。从总脂质中去除极性头部基团后，发现了两种甘油二醚核心脂质。一种是2,3 - 二 - O - 植烷基 - sn - 甘油（古菌醇），另一种是2 - O -（3'-羟基 - 3',7',11',15'-四甲基）十六烷基 - 3 - O - 植烷基 - sn - 甘油（羟基古菌醇）。这些结构是基于化学分析、快原子轰击质谱法、气 - 液色谱 - 质谱法以及1H - 和13C - NMR光谱法鉴定的。后者是一种新的核心脂质，与协同甲烷丝菌的羟基古菌醇不同。羟基古菌醇核心脂质占巴氏甲烷八叠球菌极性脂质的60%。核心脂质的结构与德罗萨等人（《生物化学与生物物理学报》（1986年）875卷，487 - 492页）先前报道的巴氏甲烷八叠球菌脂质的结构有很大不同。阐明了两种主要极性脂质的结构，这两种脂质均以羟基古菌醇作为核心成分。这些脂质是2 - O -（3'-羟基）植烷基 - 3 - O - 植烷基 - sn - 甘油 - 1 - 磷酸丝氨酸（羟基古菌磷脂酰丝氨酸）和2 - O -（3'-羟基）植烷基 - 3 - O - 植烷基 - sn - 甘油 - 磷酸 - 肌醇（羟基古菌磷脂酰 - 肌醇）。具有通常古菌醇核心部分的古菌磷脂酰丝氨酸和古菌磷脂酰肌醇也作为次要极性脂质存在。
