Patel Bhaumik B, Yu Yingjie, Du Jianhua, Levi Edi, Phillip Phillip A, Majumdar Adhip P N
Veterans Administration Medical Center, Wayne State University, Detroit, Michigan 48201, USA.
Biochem Biophys Res Commun. 2009 Jan 16;378(3):344-7. doi: 10.1016/j.bbrc.2008.10.179. Epub 2008 Nov 14.
It is becoming increasingly evident that cancer stem cells play a vital role in development and progression of cancers and relapse following chemotherapy. The present study examines the presence of cancer stem-like cells (CSC) in adenomatous polyps and in normal appearing colonic mucosa in humans during aging. The number of polyps was found to increase linearly with advancing age (r(2)=0.92, p<0.02). Immunohistochemical analysis revealed co-localization of CSC markers CD44 and CD166 in colonic polyps. Real-time RT-PCR analysis of normal appearing mucosa from subjects with adenomatous polyps showed an age-related rise in CSC as evidenced by the increased expression of CD44, CD166 and ESA. A similar phenomenon was also observed for EGFR. In addition, the expression each CSC marker was found to be about 2-fold higher in subjects with 3-4 polyps than those with 1-2 polyps. In conclusion, our results show that colon cancer stem-like cells are present in the premalignant adenomatous polyps as well in normal appearing colonic mucosa. Moreover, our observation of the age-related rise in CSC in macroscopically normal colonic mucosa suggests a predisposition of the organ to developing colorectal cancer.
越来越明显的是，癌症干细胞在癌症的发生、发展以及化疗后的复发中起着至关重要的作用。本研究检测了人类衰老过程中腺瘤性息肉及外观正常的结肠黏膜中癌症干细胞样细胞（CSC）的存在情况。发现息肉数量随年龄增长呈线性增加（r(2)=0.92，p<0.02）。免疫组织化学分析显示CSC标志物CD44和CD166在结肠息肉中共定位。对患有腺瘤性息肉的受试者外观正常的黏膜进行实时RT-PCR分析表明，CSC随年龄增长而增加，CD44、CD166和ESA的表达增加证明了这一点。表皮生长因子受体（EGFR）也观察到类似现象。此外，发现有3 - 4个息肉的受试者中每个CSC标志物的表达比有1 - 2个息肉的受试者高约2倍。总之，我们的结果表明，癌前腺瘤性息肉以及外观正常的结肠黏膜中存在结肠癌干细胞样细胞。此外，我们观察到宏观上正常的结肠黏膜中CSC随年龄增长而增加，这表明该器官易患结直肠癌。
