Rahim Gwendoline, Bischof Sylvain, Kessler Felix, Agne Birgit
Laboratoire de Physiologie Végétale, Institut de Biologie, Université de Neuchâtel, Rue Emile-Argand 11, CH-2009 Neuchâtel, Switzerland.
J Exp Bot. 2009;60(1):257-67. doi: 10.1093/jxb/ern283. Epub 2008 Nov 14.
The GTPases atToc33 and atToc159 are pre-protein receptor components of the translocon complex at the outer chloroplast membrane in Arabidopsis. Despite their participation in the same complex in vivo, evidence for their interaction is still lacking. Here, a split-ubiquitin system is engineered for use in plants, and the in vivo interaction of the Toc GTPases in Arabidopsis and tobacco protoplasts is shown. Using the same method, the self-interaction of the peroxisomal membrane protein atPex11e is demonstrated. The finding suggests a more general suitability of the split-ubiquitin system as a plant in vivo interaction assay.
GTP 酶 atToc33 和 atToc159 是拟南芥叶绿体外膜上转运体复合物的前体蛋白受体成分。尽管它们在体内参与同一个复合物，但仍缺乏它们相互作用的证据。在此，构建了一种用于植物的分裂泛素系统，并展示了拟南芥和烟草原生质体中 Toc GTP 酶的体内相互作用。使用相同方法，证明了过氧化物酶体膜蛋白 atPex11e 的自我相互作用。这一发现表明分裂泛素系统作为一种植物体内相互作用检测方法具有更广泛的适用性。
