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番茄叶霉病菌中六个疏水蛋白的沉默：同时靶向多个基因的复杂性

Silencing of six hydrophobins in Cladosporium fulvum: complexities of simultaneously targeting multiple genes.

作者信息

Lacroix Hélène, Spanu Pietro D

机构信息

Department of Life Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, United Kingdom.

出版信息

Appl Environ Microbiol. 2009 Jan;75(2):542-6. doi: 10.1128/AEM.01816-08. Epub 2008 Nov 14.

DOI:10.1128/AEM.01816-08
PMID:19011063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2620718/
Abstract

In this study, we have constructed and expressed inverted repeat chimeras from the first exons of the six known hydrophobins of the fungus Cladosporium fulvum, the causal agent of tomato leaf mold. We used quantitative PCR to measure specifically the expression levels of the hydrophobins. The targeted genes are silenced to different degrees, but we also detected clear changes in the expression levels of nontargeted genes. This work highlights the difficulties that are likely to be encountered when attempting to silence more than one gene in a multigene family.

摘要

在本研究中，我们构建并表达了来自番茄叶霉病病原菌——黄枝孢菌六种已知疏水蛋白第一个外显子的反向重复嵌合体。我们使用定量PCR专门测量疏水蛋白的表达水平。靶向基因在不同程度上被沉默，但我们也检测到非靶向基因表达水平的明显变化。这项工作突出了在多基因家族中尝试沉默多个基因时可能遇到的困难。

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本文引用的文献

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2
RNA interference: the story of gene silencing in plants and humans.RNA干扰：植物和人类基因沉默的故事
Biotechnol Adv. 2008 May-Jun;26(3):202-9. doi: 10.1016/j.biotechadv.2007.12.002. Epub 2007 Dec 23.
3
RNA silencing platforms in plants.植物中的RNA沉默平台。
FEBS Lett. 2005 Oct 31;579(26):5982-7. doi: 10.1016/j.febslet.2005.08.014. Epub 2005 Aug 22.
4
RNA silencing in fungi: mechanisms and applications.真菌中的RNA沉默：机制与应用
FEBS Lett. 2005 Oct 31;579(26):5950-7. doi: 10.1016/j.febslet.2005.08.016. Epub 2005 Aug 24.
5
Tandem inverted repeat system for selection of effective transgenic RNAi strains in Chlamydomonas.用于在衣藻中筛选有效转基因RNA干扰菌株的串联反向重复系统。
Plant J. 2004 Nov;40(4):611-21. doi: 10.1111/j.1365-313X.2004.02227.x.
6
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7
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Fungal Genet Biol. 2004 Oct;41(10):963-71. doi: 10.1016/j.fgb.2004.06.006.
8
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FEMS Microbiol Lett. 2004 Aug 15;237(2):317-24. doi: 10.1016/j.femsle.2004.06.048.
9
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Mol Microbiol. 2004 Jul;53(1):153-65. doi: 10.1111/j.1365-2958.2004.04131.x.
10
Effectiveness of RNA interference in transgenic plants.RNA干扰在转基因植物中的有效性。
FEBS Lett. 2004 May 21;566(1-3):223-8. doi: 10.1016/j.febslet.2004.04.043.