Spanu P
Department of Plant Sciences, University of Oxford, UK.
Gene. 1997 Jul 1;193(1):89-96. doi: 10.1016/s0378-1119(97)00090-5.
A hydrophobin, named HCF-1, was isolated from the culture medium of Cladosporium fulvum, the causal agent of tomato leaf mould. The protein forms insoluble aggregates when the medium is vigorously aerated. These aggregates can be dissociated by trifluoroacetic acid into monomers which migrate as 10-kDa molecules on SDS-PAGE. HCf-1 is encoded by a single gene, HCf-1. The gene contains two small introns and is translated into a 105-amino acid protein which is then processed to give a mature 83-amino acid protein. The position of the eight cysteine residues and the predicted hydrophobicity profile are typical of fungal hydrophobins. HCf-1 RNA is expressed in growing mycelium and conidia but its quantity diminishes transiently after germination; its abundance does not change when the fungus is grown on nitrogen- or carbon-deficient medium. This is the first step in evaluating the role of hydrophobins in establishment of basic compatibility between C. fulvum and tomato.
从番茄叶霉病的病原菌——番茄褐孢霉的培养基中分离出一种名为HCF-1的疏水蛋白。当培养基剧烈通气时，该蛋白会形成不溶性聚集体。这些聚集体可被三氟乙酸解离成单体，在SDS-PAGE上以10 kDa分子的形式迁移。HCf-1由单个基因HCf-1编码。该基因包含两个小内含子，被翻译成一个105个氨基酸的蛋白质，随后经过加工形成一个成熟的83个氨基酸的蛋白质。八个半胱氨酸残基的位置和预测的疏水性图谱是真菌疏水蛋白的典型特征。HCf-1 RNA在生长的菌丝体和分生孢子中表达，但在萌发后其数量会短暂减少；当真菌在氮或碳缺乏的培养基上生长时，其丰度不会改变。这是评估疏水蛋白在番茄褐孢霉与番茄之间基本相容性建立过程中作用的第一步。
