Jiang Hong, Wang Rong, Liu Yan, Zhang Yun, Chen Zhe-Yu
Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.
Clin Chim Acta. 2009 Feb;400(1-2):3-7. doi: 10.1016/j.cca.2008.10.017. Epub 2008 Oct 30.
Brain-derived neurotrophic factor (BDNF) is involved in the pathophysiology of coronary artery disease (CAD). The human BDNF Val66Met polymorphism has been shown to be associated with altered susceptibility to neuropsychiatric disorders. However it is unknown whether this polymorphism plays a role in cardiovascular disease.
Genotyping of BDNF Val66Met polymorphism was carried out in 513 controls, 628 unstable angina pectoris (UAP) and 276 stable angina pectoris (SAP) patients. The plasma concentrations of BDNF and high-sensitivity C-reactive protein (hsCRP) were measured by ELISA. The general clinical data in patients and controls were obtained.
There was a significant association between genotype and allele frequency of the BDNF Val66Met polymorphism and UAP (all P<0.05). Multivariate logistic regression analysis revealed that the BDNF(Met/Met) genotype had a protective effect on the occurrence of UAP after controlling for known risk factors of CAD (OR 0.53, P=0.005). Subjects with BDNF(Met/Met) genotype also had decreased plasma hsCRP levels compared with the Val carriers (P<0.01).
The BDNF(Met/Met) genotype has a protective effect on the occurrence of UAP, which might in part be due to the decreased plasma hsCRP level in BDNF(Met/Met) carriers. To our knowledge, this is the first study that demonstrates the link between BDNF Val66Met polymorphism and CAD.
脑源性神经营养因子（BDNF）参与冠状动脉疾病（CAD）的病理生理过程。人类BDNF Val66Met多态性已被证明与神经精神疾病易感性改变有关。然而，这种多态性是否在心血管疾病中起作用尚不清楚。
对513名对照者、628名不稳定型心绞痛（UAP）患者和276名稳定型心绞痛（SAP）患者进行BDNF Val66Met多态性基因分型。采用酶联免疫吸附测定法（ELISA）检测血浆BDNF和高敏C反应蛋白（hsCRP）浓度。获取患者和对照者的一般临床资料。
BDNF Val66Met多态性的基因型和等位基因频率与UAP之间存在显著关联（均P<0.05）。多因素逻辑回归分析显示，在控制CAD已知危险因素后，BDNF（Met/Met）基因型对UAP的发生具有保护作用（比值比0.53，P=0.005）。与携带Val等位基因者相比，BDNF（Met/Met）基因型受试者的血浆hsCRP水平也降低（P<0.01）。
BDNF（Met/Met）基因型对UAP的发生具有保护作用，这可能部分归因于BDNF（Met/Met）携带者血浆hsCRP水平降低。据我们所知，这是第一项证明BDNF Val66Met多态性与CAD之间联系的研究。
