甘珀酸对大鼠海马结构θ节律的影响：体外和体内研究方法

The effect of carbenoxolone on hippocampal formation theta rhythm in rats: in vitro and in vivo approaches.

作者信息

Bocian Renata, Posłuszny Anna, Kowalczyk Tomasz, Gołebiewski Henryk, Konopacki Jan

机构信息

Department of Neurobiology, The University of Łódź, Łódź 90-222, Rewolucji 1905 st. No. 66, Poland.

出版信息

Brain Res Bull. 2009 Mar 30;78(6):290-8. doi: 10.1016/j.brainresbull.2008.10.005. Epub 2008 Nov 14.

DOI:10.1016/j.brainresbull.2008.10.005

PMID:19013505

Abstract

The role of gap junction (GJ) coupling in the generation of hippocampal formation theta rhythm was investigated in vitro, with use of brain slices, and in vivo, with use of urethane anesthetized rats. Carbenoxolone, the succinyl ester of glycyrrhetinic acid, and GJ blocker reversibly abolished hippocampal formation theta rhythm recorded in slice preparations and urethane anesthetized rats. The present study yielded novel data which demonstrated that the pattern of delay in blockage of theta rhythm after carbenoxolone treatment, and the pattern of theta recovery after administration of this agent, require a specific time period (2-3h for delay and 8-12h for recovery), one that can be demonstrated using different experimental protocols.

摘要

利用脑片在体外以及利用氨基甲酸乙酯麻醉的大鼠在体内研究了缝隙连接（GJ）耦联在海马结构θ节律产生中的作用。甘草次酸的琥珀酸酯——生胃酮，一种GJ阻滞剂，可逆性地消除了脑片制备物和氨基甲酸乙酯麻醉大鼠中记录到的海马结构θ节律。本研究产生了新的数据，表明生胃酮处理后θ节律阻断延迟的模式以及该药物给药后θ节律恢复的模式需要特定的时间段（延迟2 - 3小时，恢复8 - 12小时），这一时间段可以通过不同的实验方案得到证明。