Leuba Sanford H, Wheeler Travis B, Cheng Chao-Min, LeDuc Philip R, Fernández-Sierra Mónica, Quiñones Edwin
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Methods. 2009 Mar;47(3):214-22. doi: 10.1016/j.ymeth.2008.10.022. Epub 2008 Nov 17.
Here we describe the use of magnetic tweezers and or microfluidics to manipulate single DNA molecules. We describe experiment which employ magnetic tweezers coupled to an inverted microscope as well as the use of a magnetic tweezers setup with an upright microscope. Using a chamber prepared via soft lithography, we also describe a microfluidic device for the manipulation of individual DNA molecules. Finally, we present some past successful examples of using these approaches to elucidate unique information about protein-nucleic acid interactions.
在这里，我们描述了使用磁镊和/或微流控技术来操纵单个DNA分子。我们描述了将磁镊与倒置显微镜耦合的实验，以及使用带有正置显微镜的磁镊装置。通过软光刻制备一个腔室，我们还描述了一种用于操纵单个DNA分子的微流控装置。最后，我们展示了一些过去使用这些方法来阐明有关蛋白质 - 核酸相互作用独特信息的成功示例。
