Jolicoeur F B, Rivest R, Drumheller A
Department of Psychiatry, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Brain Res Bull. 1991 Feb;26(2):317-20. doi: 10.1016/0361-9230(91)90245-f.
Bilateral administration of 6-hydroxydopamine in the medial fore-brain bundle at the level of the posterolateral hypothalamus in rats resulted in hypokinesia, muscular rigidity and tremor as determined by various behavioral assessment procedures. These neurological signs were accompanied by marked decreases in the concentrations of dopamine and its main metabolites dihydroxyphenylacetic acid and homovanillic acid in both striatum and nucleus accumbens. Administration of apomorphine (1 mg/kg) or L-Dopa (60 mg/kg) reversed or totally abolished the hypokinesia, rigidity and tremor in lesioned animals. Together, the present findings demonstrate that bilateral intrahypothalamic administration of 6-OHDA results in the appearance of the three cardinal symptoms of Parkinson's disease in rats. This model should prove to be valuable for both the study of the neuropathological processes underlying the neurological signs of this disease and the screening of potential antiparkinson agents.
在大鼠下丘脑后外侧水平的内侧前脑束双侧注射6-羟基多巴胺，通过各种行为评估程序测定，结果导致运动减退、肌肉僵硬和震颤。这些神经学症状伴随着纹状体和伏隔核中多巴胺及其主要代谢物二羟基苯乙酸和高香草酸浓度的显著降低。给损伤动物注射阿扑吗啡（1毫克/千克）或左旋多巴（60毫克/千克）可逆转或完全消除运动减退、僵硬和震颤。总之，目前的研究结果表明，双侧下丘脑内注射6-OHDA会导致大鼠出现帕金森病的三大主要症状。该模型对于研究这种疾病神经学症状背后的神经病理过程以及筛选潜在的抗帕金森药物都应具有重要价值。
