通过脱氧核糖核酸酶法测定环磷酸腺苷刺激的蟾蜍膀胱上皮细胞中的肌动蛋白解聚。

Actin depolymerization in the cyclic AMP-stimulated toad bladder epithelial cell, determined by the DNAse method.

作者信息

Hays R M, Lindberg U

机构信息

Albert Einstein College of Medicine, Bronx, New York 10461.

出版信息

FEBS Lett. 1991 Mar 25;280(2):397-9. doi: 10.1016/0014-5793(91)80340-9.

DOI:10.1016/0014-5793(91)80340-9

PMID:1901549

Abstract

Previous studies with the rhodamine phalloidin binding assay have shown that antidiuretic hormone and 8-Br-cAMP rapidly depolymerize F-actin in toad bladder epithelial cells. We have extended these studies with the DNAse inhibition assay and have found that in isolated epithelial cell suspensions, G-actin increases from 37 to 56% of total actin following 8-br-cAMP stimulation. The G-actin concentration in the epithelial cell greatly exceeds its critical concentration, indicating the requirement for a G-actin sequestering protein or proteins in this system.

摘要

先前使用罗丹明鬼笔环肽结合试验的研究表明，抗利尿激素和8-溴环磷酸腺苷（8-Br-cAMP）可使蟾蜍膀胱上皮细胞中的F-肌动蛋白迅速解聚。我们通过脱氧核糖核酸酶抑制试验扩展了这些研究，发现8-溴环磷酸腺苷刺激后，在分离的上皮细胞悬液中，球形肌动蛋白（G-肌动蛋白）占总肌动蛋白的比例从37%增加到56%。上皮细胞中的G-肌动蛋白浓度大大超过其临界浓度，表明该系统中需要一种或多种G-肌动蛋白隔离蛋白。