Sherman M B, Orlova E V, Smirnova E A, Hovmöller S, Zorin N A
Institute of Crystallography, USSR Academy of Sciences, Moscow.
J Bacteriol. 1991 Apr;173(8):2576-80. doi: 10.1128/jb.173.8.2576-2580.1991.
The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped complex containing six each of the large (62-kDa) and small (26-kDa) subunits. The complex is very open, with six well-separated dumbbell-shaped masses surrounding a large cylindrical hole. Each dumbbell is interpreted as consisting of one large and one small subunit.
通过对玫瑰色硫囊菌含镍氢化酶微晶进行电子显微镜观察和计算机图像处理，已确定其三维结构，在平面方向分辨率为2纳米，垂直方向分辨率为4纳米。该酶形成一个大的环形复合物，包含六个大（62 kDa）亚基和六个小（26 kDa）亚基。该复合物非常开放，有六个间隔良好的哑铃形团块围绕着一个大的圆柱形孔。每个哑铃被解释为由一个大亚基和一个小亚基组成。
