Bagyinka C, Szokefalvi-Nagy Z, Demeter I, Kovacs K L
Institute of Biophysics, Hungarian Academy of Sciences, Szeged.
Biochem Biophys Res Commun. 1989 Jul 14;162(1):422-6. doi: 10.1016/0006-291x(89)92014-7.
Polyacrylamide gel electrophoresis combined with proton induced X-ray emission spectroscopy is suitable to identify and to determine the relative amounts of protein bound metals in situ. An analysis of the hydrogenase from Thiocapsa roseopersicina has shown the feasibility of the technique and provides new insight into the relative amount as well as the intramolecular location of Fe and Ni metal atoms in this enzyme.
聚丙烯酰胺凝胶电泳与质子诱导X射线发射光谱相结合适用于原位鉴定和测定蛋白质结合金属的相对含量。对玫瑰色硫囊菌氢化酶的分析表明了该技术的可行性，并为该酶中Fe和Ni金属原子的相对含量以及分子内位置提供了新的见解。
