用于分析蓝藻鱼腥藻属Anabaena sp.菌株PCC 7120中启动子的载体。
A vector for analysis of promoters in the cyanobacterium Anabaena sp. strain PCC 7120.
作者信息
Lang J D, Haselkorn R
机构信息
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
出版信息
J Bacteriol. 1991 Apr;173(8):2729-31. doi: 10.1128/jb.173.8.2729-2731.1991.
Abstract
A plasmid vector containing a multiple-cloning site followed by a promoterless chloramphenicol acetyltransferase (cat) gene, protected by transcription terminators and mobilizable by conjugation from Escherichia coli into Anabaena sp. strain PCC 7120, was constructed. The utility of the vector was shown by deletion analysis of the promoter region of the Anabaena psbB gene.
摘要
构建了一种质粒载体，其包含一个多克隆位点，后面跟着一个无启动子的氯霉素乙酰转移酶（cat）基因，该基因受到转录终止子的保护，并且可通过接合作用从大肠杆菌转移到鱼腥藻属PCC 7120菌株中。通过对鱼腥藻psbB基因启动子区域的缺失分析，证明了该载体的实用性。
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