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用于分析蓝藻鱼腥藻属Anabaena sp.菌株PCC 7120中启动子的载体。

A vector for analysis of promoters in the cyanobacterium Anabaena sp. strain PCC 7120.

作者信息

Lang J D, Haselkorn R

机构信息

Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

出版信息

J Bacteriol. 1991 Apr;173(8):2729-31. doi: 10.1128/jb.173.8.2729-2731.1991.

DOI:10.1128/jb.173.8.2729-2731.1991
PMID:1901575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207847/
Abstract

A plasmid vector containing a multiple-cloning site followed by a promoterless chloramphenicol acetyltransferase (cat) gene, protected by transcription terminators and mobilizable by conjugation from Escherichia coli into Anabaena sp. strain PCC 7120, was constructed. The utility of the vector was shown by deletion analysis of the promoter region of the Anabaena psbB gene.

摘要

构建了一种质粒载体，其包含一个多克隆位点，后面跟着一个无启动子的氯霉素乙酰转移酶（cat）基因，该基因受到转录终止子的保护，并且可通过接合作用从大肠杆菌转移到鱼腥藻属PCC 7120菌株中。通过对鱼腥藻psbB基因启动子区域的缺失分析，证明了该载体的实用性。

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本文引用的文献

1
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J Mol Biol. 1981 May 15;148(2):107-27. doi: 10.1016/0022-2836(81)90508-8.
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Defining a bacteriophage T4 late promoter: absence of a "-35" region.界定噬菌体T4晚期启动子：不存在“-35”区域。
Cell. 1984 Jan;36(1):211-9. doi: 10.1016/0092-8674(84)90091-6.
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Organization and transcription of genes important in Anabaena heterocyst differentiation.鱼腥藻异形胞分化中重要基因的组织与转录
Ann Microbiol (Paris). 1983 Jul-Aug;134B(1):181-93. doi: 10.1016/s0769-2609(83)80104-5.
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Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120.蓝藻鱼腥藻7120 RNA聚合酶的纯化与特性分析
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6
Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogen.鱼腥藻属PCC 7120菌株中不能在有氧条件下以二氮生长的突变体的分离与互补
J Bacteriol. 1988 Mar;170(3):1239-44. doi: 10.1128/jb.170.3.1239-1244.1988.
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Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.形成异形胞的蓝细菌鱼腥藻PCC 7120菌株基因组的物理图谱和遗传图谱。
J Bacteriol. 1989 Nov;171(11):5940-8. doi: 10.1128/jb.171.11.5940-5948.1989.
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Isolation, sequence and transcription of the gene encoding the photosystem II chlorophyll-binding protein, CP-47, in the cyanobacterium Anabaena 7120.蓝藻鱼腥藻7120中光系统II叶绿素结合蛋白CP - 47编码基因的分离、测序及转录
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