Yabsley Michael J, Brown Emily L, Roellig Dawn M
Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, Georgia 30602, USA.
J Parasitol. 2009 Jun;95(3):775-7. doi: 10.1645/GE-1734.1.
An immunochromatographic assay (Chagas Stat-Pak) was evaluated for the detection of Trypanosoma cruzi antibodies in 4 species of wildlife reservoirs. Antibodies to T. cruzi were detected in raccoons (Procyon lotor) (naturally and experimentally infected) and degus (Octodon degu) (experimentally-infected) using the Chagas Stat-Pak. In naturally exposed wild raccoons, the Chagas Stat-Pak had a sensitivity and specificity of 66.7-80.0% and 96.3%, respectively. Compared with indirect immunofluorescent antibody assay results, seroconversion as determined by Chagas Stat-Pak was delayed for experimentally infected raccoons, but occurred sooner in experimentally infected degus. The Chagas Stat-Pak did not detect antibodies in naturally or experimentally infected Virginia opossums (Didelphis virginiana) or in experimentally infected short-tailed opossums (Monodelphis domestica). These data suggest that the Chagas Stat-Pak might be useful in field studies of raccoons and degus when samples would not be available for more-conventional serologic assays. Because this assay did not work on either species of marsupial, the applicability of the assay should be examined before it is used in other wild species.
对一种免疫层析检测法（查加斯快速检测试剂盒）进行了评估，以检测4种野生动物宿主中的克氏锥虫抗体。使用查加斯快速检测试剂盒在浣熊（北美浣熊）（自然感染和实验感染）和八齿鼠（实验感染）中检测到了抗克氏锥虫抗体。在自然暴露的野生浣熊中，查加斯快速检测试剂盒的灵敏度和特异性分别为66.7 - 80.0%和96.3%。与间接免疫荧光抗体检测结果相比，对于实验感染的浣熊，查加斯快速检测试剂盒确定的血清转化有所延迟，但在实验感染的八齿鼠中出现得更早。查加斯快速检测试剂盒在自然感染或实验感染的弗吉尼亚负鼠（北美负鼠）或实验感染的短尾负鼠（家短尾负鼠）中未检测到抗体。这些数据表明，当无法获得样本进行更常规的血清学检测时，查加斯快速检测试剂盒可能在浣熊和八齿鼠的野外研究中有用。由于该检测法对两种有袋动物均无效，在将其用于其他野生动物物种之前，应先检查其适用性。
