Lee Yong-Seok, Choi Sun-Lim, Kim Tae-Hyung, Lee Jin-A, Kim Hyong Kyu, Kim Hyoung, Jang Deok-Jin, Lee Jennifer J, Lee Sunghoon, Sin Gwang Sik, Kim Chang-Bae, Suzuki Yutaka, Sugano Sumio, Kubo Tai, Moroz Leonid L, Kandel Eric R, Bhak Jong, Kaang Bong-Kiun
National Creative Research Initiative, Center for Memory and Institute of Molecular Biology and Genetics, Department of Biological Sciences, College of Natural Sciences, Seoul National University, San 56-1 Silim-dong, Gwanak-gu, Seoul 151-747, Korea.
Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18602-7. doi: 10.1073/pnas.0808893105. Epub 2008 Nov 18.
The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.
海洋软体动物海兔是研究行为和可塑性的细胞基础的一种有用的模式生物。然而,海兔的分子研究一直受到基因组信息缺乏的限制。最近,对加州海兔的神经元转录本进行了大规模表征。在此,我们报告了对来自西北太平洋的一个近缘物种日本海兔的一组平行神经元转录本的分析。我们从日本海兔的神经系统组织中收集了4859个非冗余序列。通过进行微阵列和实时PCR分析,我们发现,5-羟色胺(5-HT)处理后,ApC/EBP、matrilin、抗凝血酶和eIF3e克隆显著上调,而一个BAT1同源克隆显著下调。其中,我们进一步证明,Ap-eIF3e作为一个正向调节因子在5-HT诱导的长时程易化(LTF)中起关键作用。