Conte Catherine, Herdegen Samantha, Kamal Saman, Patel Jency, Patel Ushma, Perez Leticia, Rivota Marissa, Calin-Jageman Robert J, Calin-Jageman Irina E
Neuroscience Program, Dominican University, River Forest, Illinois 60305, USA.
Learn Mem. 2017 Sep 15;24(10):502-515. doi: 10.1101/lm.045450.117. Print 2017 Oct.
We characterized the transcriptional response accompanying maintenance of long-term sensitization (LTS) memory in the pleural ganglia of using microarray ( = 8) and qPCR ( = 11 additional samples). We found that 24 h after memory induction there is strong regulation of 1198 transcripts (748 up and 450 down) in a pattern that is almost completely distinct from what is observed during memory encoding (1 h after training). There is widespread up-regulation of transcripts related to all levels of protein production, from transcription (e.g., subunits of transcription initiation factors) to translation (e.g., subunits of eIF1, eIF2, eIF3, eIF4, eIF5, and eIF2B) to activation of components of the unfolded protein response (e.g., CREB3/Luman, BiP, AATF). In addition, there are widespread changes in transcripts related to cytoskeleton function, synaptic targeting, synaptic function, neurotransmitter regulation, and neuronal signaling. Many of the transcripts identified have previously been linked to memory and plasticity (e.g., Egr, menin, TOB1, IGF2 mRNA binding protein 1/ZBP-1), though the majority are novel and/or uncharacterized. Interestingly, there is regulation that could contribute to metaplasticity potentially opposing or even eroding LTS memory (down-regulation of adenylate cyclase and a putative serotonin receptor, up-regulation of FMRFa and a FMRFa receptor). This study reveals that maintenance of a "simple" nonassociative memory is accompanied by an astonishingly complex transcriptional response.
我们使用微阵列（n = 8）和定量聚合酶链反应（qPCR，另外11个样本）对[具体生物]胸膜神经节中伴随长期敏感化（LTS）记忆维持的转录反应进行了表征。我们发现，在记忆诱导后24小时，1198个转录本受到强烈调控（748个上调，450个下调），其模式与记忆编码期间（训练后1小时）观察到的几乎完全不同。从转录（例如转录起始因子的亚基）到翻译（例如eIF1、eIF2、eIF3、eIF4、eIF5和eIF2B的亚基）再到未折叠蛋白反应成分的激活（例如CREB3/Luman、BiP、AATF），与蛋白质生产各个水平相关的转录本普遍上调。此外，与细胞骨架功能、突触靶向、突触功能、神经递质调节和神经元信号传导相关的转录本也有广泛变化。许多已鉴定的转录本先前已与记忆和可塑性相关联（例如Egr、menin、TOB1、IGF2 mRNA结合蛋白1/ZBP-1），尽管大多数是新的和/或未表征的。有趣的是，存在一些调控可能导致潜在地对抗甚至侵蚀LTS记忆的元可塑性（腺苷酸环化酶和一种假定的5-羟色胺受体下调，FMRFa和一种FMRFa受体上调）。这项研究表明，维持一种“简单”的非联想记忆伴随着惊人复杂的转录反应。
