Khrebtukova I A, Kwiatkowska K, Gudkova D A, Sorokin A B, Pinaev G P
Institute of Cytology, Academy of Sciences, Leningrad, USSR.
Exp Cell Res. 1991 May;194(1):48-55. doi: 10.1016/0014-4827(91)90128-h.
Capping of the EGF receptor (EGF-R) on the surface of suspended and adherent epidermoid carcinoma cells, A431, is studied. It was induced at 20 degrees C after treating cells with monoclonal antibody to the EGF receptor followed by the second antibody conjugated with FITC. Accumulation of cortical actin under the caps was detected by rhodamine-phalloidin. Destruction of the actin stress-fiber-like bundles was observed during incubation of cells with the ligands at 0 degrees C. Two processes appear to take place at 20 degrees C: redistribution of the EGF-R with cortical actin into the caps within 15-30 min and reconstruction of cytoplasmic actin bundles over 45-60 min. Dihydrocytochalasin B prevented cap formation in adherent cells, but small patches of EGF-R colocalized with actin aggregates under plasma membrane were observed. The function of different actin-containing cytoskeleton structures in the process of capping is discussed.
对悬浮和贴壁的表皮样癌细胞A431表面的表皮生长因子受体（EGF-R）的封帽现象进行了研究。在用抗EGF受体单克隆抗体处理细胞，随后用与异硫氰酸荧光素（FITC）偶联的二抗处理后，于20℃诱导封帽。用罗丹明-鬼笔环肽检测帽下皮质肌动蛋白的积累。在0℃将细胞与配体孵育期间，观察到肌动蛋白应力纤维样束的破坏。在20℃似乎发生两个过程：EGF-R与皮质肌动蛋白在15 - 30分钟内重新分布到帽中，以及在45 - 60分钟内细胞质肌动蛋白束的重建。二氢细胞松弛素B阻止贴壁细胞形成封帽，但观察到小的EGF-R斑块与质膜下的肌动蛋白聚集体共定位。讨论了不同含肌动蛋白的细胞骨架结构在封帽过程中的作用。
