St John Franz J, Feng Bo, Pozharski Edwin
Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore 21201, USA.
Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1222-7. doi: 10.1107/S0907444908031302. Epub 2008 Nov 18.
The automated microseeding protocol previously proposed by D'Arcy et al. [D'Arcy et al. (2007), Acta Cryst. D63, 550-554] includes an inherent chemical shift in all conditions in a sparse-matrix screen. This results from the uniform addition of mother liquor used to stabilize the microcrystalline seed stock, which is usually the mother liquor that resulted in successful crystal growth. It was found that there is an overlap in identified crystallization leads between addition of seed and addition of the mother liquor used to stabilize the seed. This indicates that additional crystallization ;hits' primarily (but not exclusively) arise from changes in the drop composition, not the introduction of seeds. A complementary automated microseeding protocol is proposed as a novel approach for biasing drops in a screen with many of the most popular precipitants.
达西等人[达西等人（2007年），《晶体学报》D63卷，550 - 554页]之前提出的自动微量接种方案在稀疏矩阵筛选的所有条件下都存在固有的化学位移。这是由于均匀添加用于稳定微晶种子储备的母液导致的，该母液通常是促成晶体成功生长的母液。研究发现，在添加种子和添加用于稳定种子的母液之间，所确定的结晶线索存在重叠。这表明额外的结晶“命中”主要（但并非唯一）源于液滴成分的变化，而非种子的引入。本文提出了一种互补的自动微量接种方案，作为在使用许多最常用沉淀剂的筛选中使液滴产生偏差的新方法。
