Pechkova Eugenia, Fiordoro Stefano, Fontani David, Nicolini Claudio
Fondazione Elba, Via delle Testuggini snc, Roma, Italy.
Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):809-12. doi: 10.1107/S0907444905006566. Epub 2005 May 26.
Protein nanocrystallography has recently been introduced as a unique nanotechnology-based approach to forming stable protein crystals and to characterize them down to atomic resolution. In particular, a protein nanostuctured template appears to be capable of stimulating nucleation and crystal growth of so far unsolved proteins. In the present communication, aimed at investigating the lysozyme crystal-growth mechanisms with and without nanotemplate, the lysozymes appear to transfer directly from the nanostructured film into the drop to trigger the formation of the crystal, therefore highlighting the physical interpretation of the mechanism for nanotemplate-induced protein crystallization.
蛋白质纳米晶体学最近作为一种独特的基于纳米技术的方法被引入，用于形成稳定的蛋白质晶体并将其表征至原子分辨率。特别是，蛋白质纳米结构模板似乎能够刺激迄今尚未解析的蛋白质的成核和晶体生长。在本通讯中，旨在研究有无纳米模板时溶菌酶的晶体生长机制，溶菌酶似乎直接从纳米结构薄膜转移到液滴中以触发晶体形成，因此突出了纳米模板诱导蛋白质结晶机制的物理解释。
