M'ghirbi Y, Ghorbel A, Amouri M, Nebaoui A, Haddad S, Bouattour A
Service d'Entomologie Médicale, Institut Pasteur de Tunis, 1002 Tunis, Tunisia.
Parasitol Res. 2009 Mar;104(4):767-74. doi: 10.1007/s00436-008-1253-4. Epub 2008 Nov 19.
A seroepidemiological survey was conducted in five bioclimatic areas of Tunisia to determine the prevalence of antibodies to Ehrlichia canis and Anaplasma phagocytophilum antigens, surrogate markers of the agents of canine monocytic ehrlichiosis (CME) and canine granulocytic ehrlichiosis, respectively. Among 286 collected sera, 54.2% and 25.2% were seropositive for E. canis and A. phagocytophilum, respectively, by the indirect immunofluorescence antibody (IFA) test. Clinical and hematological tests were done only for 58 sick dogs from Tunis area. A reverse line blot (RLB) hybridization was then used to identify isolated Ehrlichia and Anaplasma species infecting dogs (n = 228). Among them, only two dogs were infected by A. phagocytophilum; ten sample dogs were demonstrated infected by E. canis and ten infected by Ehrlichia sp., from which one dog showed a mixed infection with A. phagocytophilum and E. canis and one with A. phagocytophilum and Ehrlichia sp. RLB findings were confirmed by sequencing; BLAST search against GenBank revealed high similarity of the sequence of Ehrlichia sp. PCR/RLB amplicons with Anaplasma platys 16S rRNA partial sequence.
在突尼斯的五个生物气候区进行了一项血清流行病学调查，以确定犬埃立克体（Ehrlichia canis）和嗜吞噬细胞无形体（Anaplasma phagocytophilum）抗原抗体的流行率，这两种抗原分别是犬单核细胞埃立克体病（CME）和犬粒细胞埃立克体病病原体的替代标志物。在收集的286份血清中，通过间接免疫荧光抗体（IFA）检测，犬埃立克体和嗜吞噬细胞无形体的血清阳性率分别为54.2%和25.2%。仅对来自突尼斯地区的58只患病犬进行了临床和血液学检测。然后使用反向线印迹（RLB）杂交来鉴定感染犬的埃立克体和无形体分离株（n = 228）。其中，仅两只犬感染了嗜吞噬细胞无形体；十只样本犬被证实感染了犬埃立克体，十只感染了埃立克体属（Ehrlichia sp.），其中一只犬表现出嗜吞噬细胞无形体和犬埃立克体的混合感染，一只表现出嗜吞噬细胞无形体和埃立克体属的混合感染。RLB结果通过测序得到证实；对GenBank进行BLAST搜索发现，埃立克体属PCR/RLB扩增子序列与血小板无形体（Anaplasma platys）16S rRNA部分序列具有高度相似性。
