Cortell C, Vicente J S, Mocé E, Marco-Jiménez F, Viudes De Castro M P
Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias, Polígono La Esperanza, Segorbe (Castellón), Spain.
Reprod Domest Anim. 2010 Feb;45(1):155-9. doi: 10.1111/j.1439-0531.2008.01294.x. Epub 2008 Nov 18.
This study aims to assess the efficiency of in vivo oocyte and embryo recovery after a recombinant human FSH (rhFSH) treatment in rabbit does. Females were distributed in two experimental groups: donor does were treated with rhFSH (superovulation group) for 3 days prior to artificial insemination (embryo recovery) or ovulation induction (oocyte recovery) and does without treatment remained as the control group. Mature oocytes or embryos were collected with the laparoscopy technique 16 h after ovulation induction (oocytes) or 72 h after artificial insemination (embryos). Up to four recoveries were performed with each doe. Recovery efficiencies differed significantly between embryos (84%) and oocytes (58%). Yet, the recovery rates for the superovulation and control groups did not differ. The rhFSH group was associated with a significant increase (p < 0.05) in the number of oocytes and embryos recovered in comparison with the control group (10.2 +/- 1.0 and 14.3 +/- 1.2 vs 6.0 +/- 2.7 and 8.4 +/- 2.3 for oocytes and embryos, respectively). Results from this study indicate that repeated in vivo oocyte and embryo recovery from rhFSH superovulated does maximizes the number of oocytes or embryos collected from the same female.
本研究旨在评估重组人促卵泡激素（rhFSH）处理后，兔体内卵母细胞和胚胎回收的效率。雌性兔子被分为两个实验组：供体兔在人工授精（胚胎回收）或排卵诱导（卵母细胞回收）前3天用rhFSH处理（超排卵组），未处理的兔子作为对照组。在排卵诱导后16小时（卵母细胞）或人工授精后72小时（胚胎），采用腹腔镜技术收集成熟的卵母细胞或胚胎。每只兔子最多进行四次回收。胚胎（84%）和卵母细胞（58%）的回收效率差异显著。然而，超排卵组和对照组的回收率没有差异。与对照组相比，rhFSH组回收的卵母细胞和胚胎数量显著增加（p < 0.05）（卵母细胞分别为10.2±1.0和14.3±1.2，胚胎分别为6.0±2.7和8.4±2.3）。本研究结果表明，从rhFSH超排卵的兔子中反复进行体内卵母细胞和胚胎回收，可使从同一雌性收集的卵母细胞或胚胎数量最大化。
