Yang Shihua, He Xiechao, Hildebrandt Thomas B, Zhou Qi, Ji Weizhi
Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan, PR China.
Am J Primatol. 2007 Nov;69(11):1278-84. doi: 10.1002/ajp.20433.
To simplify the procedure for superovulation in the rhesus monkey, this study was designed using polyvinylpyrrolidone (PVP) solution as a solvent for gonadotropins. Thirty-five cycling females (aged 5-8 years old) were divided into six groups during the breeding season (November- February). The groups were as follows: Group I, animals received twice-daily 35 IU recombinant human follicle-stimulating hormone (rhFSH) dissolved in 0.5 ml saline for 8 days as the control; Groups II and III, animals received single-daily 35 IU and 17 IU rhFSH in 0.5 saline, respectively, for 9 days; Groups IV, V and VI, received single-daily injection of 35 IU rhFSH, 17 IU rhFSH and 8.5 IU rhFSH dissolved in 0.5 ml 30% PVP (w/v) solution, respectively, for 9 days. After human chorionic gonadotropin was administered to induce the nuclear maturation of oocytes, oocytes were retrieved and the development competence of recovered oocytes treated with in vitro fertilization were observed. The plasma concentrations of follicle-stimulating hormone and ovarian responses were monitored during the treatment. The results showed that the number of recovered oocytes and the in vitro developmental competence of mature oocytes was equivalent among monkeys when treated with a single-daily treatment of 17 and 35 IU rhFSH with PVP preparation in Groups IV and V compared with the twice-daily 35 IU rhFSH treatments received by Group I. However, almost all animals in Groups II, III and VI responded poorly to corresponding stimulations. These findings indicate that a single-daily low dose of rhFSH dissolved in PVP solution can induce the satisfactory ovarian responses in rhesus monkeys. This has the potential to reduce treatment distress, stress to the animals, the labor of the operator as well as the amount of rhFSH used in ovarian stimulation, compared with traditional superovulation methods.
为简化恒河猴超数排卵程序，本研究设计使用聚乙烯吡咯烷酮（PVP）溶液作为促性腺激素的溶剂。在繁殖季节（11月至2月），将35只处于发情周期的雌性恒河猴（年龄5 - 8岁）分为六组。分组如下：第一组，动物每天两次接受溶解于0.5 ml生理盐水中的35 IU重组人促卵泡激素（rhFSH），共8天作为对照；第二组和第三组，动物分别每天一次接受溶解于0.5 ml生理盐水中的35 IU和17 IU rhFSH，共9天；第四组、第五组和第六组，分别每天一次接受溶解于0.5 ml 30% PVP（w/v）溶液中的35 IU rhFSH、17 IU rhFSH和8.5 IU rhFSH，共9天。在注射人绒毛膜促性腺激素诱导卵母细胞核成熟后，采集卵母细胞，并观察经体外受精处理的回收卵母细胞的发育能力。在治疗期间监测促卵泡激素的血浆浓度和卵巢反应。结果显示，与第一组每天两次接受35 IU rhFSH治疗相比，第四组和第五组中每天一次用PVP制剂给予17 IU和35 IU rhFSH治疗的恒河猴，回收的卵母细胞数量和成熟卵母细胞的体外发育能力相当。然而，第二组、第三组和第六组中几乎所有动物对相应刺激反应不佳。这些发现表明，每天一次低剂量溶解于PVP溶液中的rhFSH可在恒河猴中诱导出令人满意的卵巢反应。与传统超数排卵方法相比，这有可能减少治疗痛苦、动物应激、操作人员的工作量以及卵巢刺激中rhFSH的用量。
