Lei M, Xue C-H, Wang Y-M, Li Z-J, Xue Y, Wang J-F
College of Food Science and Technology, Ocean Univ. of China, Qingdao 266003, China.
J Food Sci. 2008 Oct;73(8):H207-11. doi: 10.1111/j.1750-3841.2008.00930.x.
The present study was designed to evaluate the effect of a new iron tonic (squid ink melanin-Fe [SM-Fe]) on remission of iron deficiency anemia (IDA) using a rat model of IDA. The rat IDA model was established with low-iron diet feeding and caudal vein blooding. Then different dosages of SM-Fe were given to the rats once a day by intragastric administration, with FeSO4 and FeCl3 as positive control. The content of Hemoglobin (Hb), red blood cell (RBC), hematocrit (HCT), and mean corpuscular volume (MCV) were analyzed in addition to the contents of serum iron (SI) and intracellular free erythrocyte protoporphyrin (FEP). The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum was also measured. The results showed that anemia caused by iron deficiency was established as a consequence of the low-iron diets. SM-Fe showed an effective restoration action by returning Hb, RBC, HCT, MCV, SI, and FEP in IDA animals to normal values. An antioxidant effect was also observed that reduced MDA level, enhanced the activities of SOD and GSH-Px in serum, and protected erythrocytes from the injury of reactive oxygen species as a consequence of SM-Fe intake. In comparison with FeSO4 and FeCl3, higher bioavailability of iron and fewer side effects were also observed. In conclusion, SM-Fe remitted iron deficiency anemia symptoms significantly, suggesting that SM-Fe might contribute to improving hemopoietic function in IDA rats and might be exploited as a safe, efficient new iron tonic.
本研究旨在使用缺铁性贫血（IDA）大鼠模型评估一种新型补铁剂（鱿鱼墨黑色素-铁[SM-Fe]）对缺铁性贫血缓解的影响。通过低铁饮食喂养和尾静脉放血建立大鼠IDA模型。然后通过灌胃给药，每天给大鼠一次不同剂量的SM-Fe，以硫酸亚铁和氯化铁作为阳性对照。除了分析血清铁（SI）和细胞内游离红细胞原卟啉（FEP）的含量外，还分析了血红蛋白（Hb）、红细胞（RBC）、血细胞比容（HCT）和平均红细胞体积（MCV）的含量。还测量了血清中丙二醛（MDA）的含量以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。结果表明，缺铁饮食导致了缺铁性贫血的发生。SM-Fe通过使IDA动物的Hb、RBC、HCT、MCV、SI和FEP恢复到正常水平，显示出有效的恢复作用。还观察到抗氧化作用，即降低MDA水平，增强血清中SOD和GSH-Px的活性，并保护红细胞免受因摄入SM-Fe而产生的活性氧的损伤。与硫酸亚铁和氯化铁相比，还观察到铁的生物利用度更高且副作用更少。总之，SM-Fe显著缓解了缺铁性贫血症状，表明SM-Fe可能有助于改善IDA大鼠的造血功能，并且可能被开发为一种安全、高效的新型补铁剂。
