水痘带状疱疹病毒（VZV）野生型基因型在北欧和南欧的分布：流行基因型高度保守的证据

Distribution of varicella-zoster virus (VZV) wild-type genotypes in northern and southern Europe: evidence for high conservation of circulating genotypes.

作者信息

Loparev Vladimir N, Rubtcova Elena N, Bostik Vanda, Tzaneva Valentina, Sauerbrei Andreas, Robo Alma, Sattler-Dornbacher Eva, Hanovcova Iva, Stepanova Vera, Splino Miroslav, Eremin Vladimir, Koskiniemi Marjaleena, Vankova Olga E, Schmid D Scott

机构信息

National VZV Laboratory, Centers for Disease Control and Prevention, Coordinating Center for Infectious Diseases, National Center for Preparedness, Detection, and Control of Infectious Diseases, Atlanta, GA, USA.

出版信息

Virology. 2009 Jan 20;383(2):216-25. doi: 10.1016/j.virol.2008.10.026. Epub 2008 Nov 20.

DOI:10.1016/j.virol.2008.10.026

PMID:19019403

Abstract

Phylogenetic analysis of 19 complete VZV genomic sequences resolves wild-type strains into 5 genotypes (E1, E2, J, M1, and M2). Complete sequences for M3 and M4 strains are unavailable, but targeted analyses of representative strains suggest they are stable, circulating VZV genotypes. Sequence analysis of VZV isolates identified both shared and specific markers for every genotype and validated a unified VZV genotyping strategy. Despite high genotype diversity no evidence for intra-genotypic recombination was observed. Five of seven VZV genotypes were reliably discriminated using only four single nucleotide polymorphisms (SNP) present in ORF22, and the E1 and E2 genotypes were resolved using SNP located in ORF21, ORF22 or ORF50. Sequence analysis of 342 clinical varicella and zoster specimens from 18 European countries identified the following distribution of VZV genotypes: E1, 221 (65%); E2, 87 (25%); M1, 20 (6%); M2, 3 (1%); M4, 11 (3%). No M3 or J strains were observed.

摘要

对19个完整的水痘带状疱疹病毒（VZV）基因组序列进行系统发育分析，可将野生型毒株分为5种基因型（E1、E2、J、M1和M2）。目前尚无M3和M4毒株的完整序列，但对代表性毒株的靶向分析表明它们是稳定的、正在传播的VZV基因型。对VZV分离株的序列分析确定了每种基因型的共有标记和特异性标记，并验证了统一的VZV基因分型策略。尽管基因型多样性很高，但未观察到基因型内重组的证据。仅使用开放阅读框22（ORF22）中存在的四个单核苷酸多态性（SNP）就能可靠地区分七种VZV基因型中的五种，而E1和E2基因型则通过位于ORF21、ORF22或ORF50中的SNP来区分。对来自18个欧洲国家的342份临床水痘和带状疱疹标本进行的序列分析确定了VZV基因型的以下分布：E1型221份（65%）；E2型87份（25%）；M1型20份（6%）；M2型3份（1%）；M4型11份（3%）。未观察到M3或J型毒株。