Shallal Mohammed Jasim Mohammed, Nasser Hind Ali, Naif Alaa Abdul Hassen
Department of Microbiology, College of Medicine, University of Thi-Qar, Thi-Qar, Iraq.
Department of Dermatology, College of Medicine, University of Thi-Qar, Thi-Qar, Iraq.
Iran J Microbiol. 2024 Aug;16(4):524-535. doi: 10.18502/ijm.v16i4.16312.
Herpes zoster, or shingles, is caused by the varicella-zoster virus (VZV), which initially presents as chickenpox in children. VZV is a global health concern, especially in winter and spring, affecting 10-20% of adults over 50 and posing a 30% risk for the general population. This study used PCR to detect VZV, confirming results with duplicated DNA samples and identifying 234 bp fragments by targeting the gpB gene.
This study examined 50 herpes zoster cases from October 2020 to April 2021, involving 30 males and 20 females aged 10 to 90, diagnosed by dermatologists. Data were collected via a questionnaire. PCR detected VZV by amplifying the gpB and MCP genes from skin lesion samples. Six positive 234-bp PCR products were sequenced at Macrogen Inc. in Seoul, South Korea.
Six DNA samples with 234 bp amplicons were sequenced, showing 99-100% similarity to human alpha herpesvirus sequences in the gpB gene. NCBI BLAST matched these sequences to a reference (GenBank acc. MT370830.1), assigning accession numbers LC642111, LC642112, and LC642113. Eight nucleic acid substitutions caused amino acid changes in the gpB protein: isoleucine to threonine, serine to isoleucine, and threonine to Proline. These variants were deposited in NCBI GenBank as gpB3 samples.
The study found high sequence similarity to known VZV sequences, identifying six nucleic acid variations and eight SNPs. Notable amino acid changes in the gpB protein were deposited in NCBI GenBank as the gpB3 sample.
带状疱疹，即缠腰龙，由水痘-带状疱疹病毒（VZV）引起，该病毒最初在儿童中表现为水痘。VZV是一个全球健康问题，尤其在冬春季节，影响着50岁以上10%-20%的成年人，且普通人群有30%的感染风险。本研究采用聚合酶链反应（PCR）检测VZV，通过重复DNA样本确认结果，并通过靶向糖蛋白B（gpB）基因鉴定234碱基对片段。
本研究检查了2020年10月至2021年4月期间的50例带状疱疹病例，涉及年龄在10至90岁之间的30名男性和20名女性，由皮肤科医生诊断。通过问卷调查收集数据。PCR通过扩增皮肤病变样本中的gpB和主要衣壳蛋白（MCP）基因来检测VZV。六个阳性的234碱基对PCR产物在韩国首尔的Macrogen公司进行测序。
对六个具有234碱基对扩增子的DNA样本进行了测序, 结果显示其在gpB基因中与人类α疱疹病毒序列有99%-100%的相似性。美国国立医学图书馆（NCBI）的基本局部比对搜索工具（BLAST）将这些序列与一个参考序列（GenBank登录号MT370830.1）进行匹配，分配的登录号为LC642111、LC642112和LC642113。八个核酸替换导致了gpB蛋白中的氨基酸变化：异亮氨酸变为苏氨酸、丝氨酸变为异亮氨酸、苏氨酸变为脯氨酸。这些变体作为gpB3样本存入NCBI基因库。
该研究发现与已知VZV序列具有高度的序列相似性，鉴定出六个核酸变异和八个单核苷酸多态性（SNP）。gpB蛋白中显著的氨基酸变化作为gpB3样本存入NCBI基因库。
