Departamento de Genoma de Patógenos, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Carpio 470, Mexico City 11340, Mexico.
J Clin Microbiol. 2010 May;48(5):1712-5. doi: 10.1128/JCM.00112-10. Epub 2010 Mar 10.
Full-length genome analysis of varicella-zoster virus (VZV) has shown that viral strains can be classified into seven different genotypes: European (E), Mosaic (M), and Japanese (J), and the E and M genotypes can be further subclassified into E1, E2, and M1 through 4, respectively. The distribution of the main VZV genotypes in Mexico was described earlier, demonstrating the predominance of E genotype, although other genotypes (M1 and M4) were also identified. However, no information regarding the circulation of either E genotype in the country is available. In the present study, we confirm the presence of both E1 and E2 genotypes in the country and explore the possibility of coinfection as the triggering factor for increased virulence among severe cases. A total of 61 different European VZV isolates collected in the Mexico City metropolitan area from 2005 to 2006 were typed by using a PCR method based on genotype-specific primer amplification. Fifty isolates belonged to the E1 genotype, and the eleven remaining samples were classified as E2 genotypes. No coinfection with both E genotypes was identified among these specimens. We provide here new information on the distribution of VZV genotypes circulating in Mexico City.
对水痘带状疱疹病毒（VZV）全长基因组的分析表明，病毒株可分为七种不同的基因型：欧洲型（E）、嵌合型（M）和日本型（J），E 和 M 基因型可进一步通过 4 分别细分为 E1、E2 和 M1。此前曾描述过墨西哥主要 VZV 基因型的分布，表明 E 基因型占主导地位，尽管也鉴定出了其他基因型（M1 和 M4）。然而，关于该国 E 基因型的流行情况尚无信息。在本研究中，我们证实了该国同时存在 E1 和 E2 基因型，并探讨了合并感染作为严重病例毒力增加的触发因素的可能性。我们使用基于基因型特异性引物扩增的 PCR 方法对 2005 年至 2006 年间在墨西哥城大都市区收集的 61 种不同的欧洲 VZV 分离株进行了分型。其中 50 株属于 E1 基因型，其余 11 个样本被归类为 E2 基因型。在这些样本中未发现两种 E 基因型的合并感染。我们在此提供了有关在墨西哥城流行的 VZV 基因型分布的新信息。
