Raftery A T
J Anat. 1976 Jul;121(Pt 3):589-97.
The healing of parietal and visceral peritoneum has been studied by the techniques of enzyme histochemistry in an attempt to define more precisely the type of cell responsibile for forming the new mesothelium. The changes occurring in the distribution of the following enzymes throughout the course of healing have been investigated: acid phosphatase, alkaline phosphatase, ATPase and non-specific esterase. Regenerating mesothelial cells have been found to have several enzyme histochemical properties in common with subperitoneal connective tissue cells. It has not been possible to distinguish between primitive mesenchymal cells and subperitoneal fibroblasts by the histochemical techniques used in this study and therefore the study has not been fruitful in determining whether the new mesothelium arises from primitive mesenchymal cells or subperitoneal fibroblasts. The present study does, however, lend weight to the view that the new mesothelium is not derived from peritoneal macrophages.
为了更精确地确定形成新间皮的细胞类型，采用酶组织化学技术对壁腹膜和脏腹膜的愈合进行了研究。对以下酶在愈合过程中分布的变化进行了研究：酸性磷酸酶、碱性磷酸酶、三磷酸腺苷酶和非特异性酯酶。已发现再生间皮细胞具有与腹膜下结缔组织细胞相同的几种酶组织化学特性。通过本研究中使用的组织化学技术，无法区分原始间充质细胞和腹膜下成纤维细胞，因此该研究在确定新间皮是否源自原始间充质细胞或腹膜下成纤维细胞方面没有取得成果。然而，本研究确实支持了新间皮并非源自腹膜巨噬细胞这一观点。
