Da Costa S C, Calabrese K S, Bauer P G, Savino W, Lagrange P H
Department of Protozoology, Institute Oswaldo Cruz, Rio-de-Janeiro, Brazil.
Pathol Biol (Paris). 1991 Feb;39(2):91-7.
Among newborn and adult euthymic and athymic mice infected intraperitoneally with 10(2) or 10(4) trypomastigotes, neonates and adult athymic animals exhibited greater susceptibility with heavier colonization of central and peripheral lymphoid organs. Contiguity was also found to be a significant factor in colonization: after similar inocula, the parasitic load in the thymus was comparable in adults and neonates when the former were infected by subcutaneous injection in the anterior neck. In the nude athymic mice, the Y (reticulotropic) strain and the CL (myotropic) strain exhibited similar invasiveness, suggesting that the host immune response modulated the tissular tropism of these strains seen in the heterozygous littermates (Nu/+). The bone marrow was heavily infected; parasites were found in chondrocytes and cells of the mononucleate phagocytic system. Ultrastructural studies of infected thymus specimens showed that the corticomedullary architecture was unaltered and that both macrophages and epithelial cells were infected: both these cell types were infected in vitro.
在经腹腔注射10²或10⁴个锥鞭毛体感染的新生和成年的正常胸腺及无胸腺小鼠中，新生小鼠和成年无胸腺动物表现出更高的易感性，中枢和外周淋巴器官的定植更严重。还发现毗邻性是定植的一个重要因素：接种相似剂量后，当成年小鼠通过前颈部皮下注射感染时，其胸腺中的寄生虫负荷与新生小鼠相当。在裸无胸腺小鼠中，Y（亲网状细胞）株和CL（亲肌细胞）株表现出相似的侵袭性，这表明宿主免疫反应调节了在杂合子同窝小鼠（Nu/+）中观察到的这些菌株的组织嗜性。骨髓受到严重感染；在软骨细胞和单核吞噬系统的细胞中发现了寄生虫。对感染胸腺标本的超微结构研究表明，皮质髓质结构未改变，巨噬细胞和上皮细胞均被感染：这两种细胞类型在体外均被感染。
