Yang K, Wen J, Liu X, Kijlstra A, Chen L, Chi W, Zhou H, Huang X, Yang P
The First Clinical Hospital of ZhengZhou University, ZhengZhou, PR China.
Br J Ophthalmol. 2009 Feb;93(2):249-53. doi: 10.1136/bjo.2008.142489. Epub 2008 Nov 19.
To evaluate the effect of rapamycin (RAPA) and dexamethasone (DEX) on the production of IL-17 and IFN-gamma by peripheral blood mononuclear cells (PBMCs) from Vogt-Koyanagi-Harada (VKH) patients and healthy individuals.
Blood samples were drawn from 10 active VKH patients and 10 healthy individuals. PBMCs were cultured with or without anti-CD3 and anti-CD28 antibodies in the presence or absence of different concentrations of RAPA or DEX for 72 h. IL-17 and IFN-gamma concentrations in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA).
The expression of IL-17 and IFN-gamma was significantly increased in active VKH patients compared with that in healthy controls. Both RAPA and DEX were able to significantly inhibit the production of IL-17 and IFN-gamma by PBMCs from patients and healthy controls. RAPA was able to completely inhibit IL-17 production at a dosage of 10 ng/ml but only partially suppressed IFN-gamma production even at a much higher concentration (1000 ng/ml). DEX inhibited the production of both IL-17 and IFN-gamma by approximately 70%.
This study indicates that both RAPA and DEX inhibit the production of IL-17 and IFN-gamma by PBMCs. RAPA is much stronger in inhibiting the production of IL-17 than DEX.
评估雷帕霉素（RAPA）和地塞米松（DEX）对葡萄膜炎-小柳原田病（VKH）患者及健康个体外周血单个核细胞（PBMC）产生白细胞介素-17（IL-17）和干扰素-γ（IFN-γ）的影响。
采集10例活动期VKH患者和10例健康个体的血样。PBMC在有或无抗CD3和抗CD28抗体的情况下，于不同浓度的RAPA或DEX存在或不存在时培养72小时。通过酶联免疫吸附测定（ELISA）测量上清液中IL-17和IFN-γ的浓度。
与健康对照相比，活动期VKH患者中IL-17和IFN-γ的表达显著增加。RAPA和DEX均能显著抑制患者和健康对照的PBMC产生IL-17和IFN-γ。RAPA在剂量为10 ng/ml时能够完全抑制IL-17的产生，但即使在更高浓度（1000 ng/ml）时也只能部分抑制IFN-γ的产生。DEX抑制IL-17和IFN-γ的产生约70%。
本研究表明，RAPA和DEX均抑制PBMC产生IL-17和IFN-γ。RAPA在抑制IL-17产生方面比DEX更强。
