Scarfi M R, Bersani F, Cossarizza A, Monti D, Castellani G, Cadossi R, Franceschetti G, Franceschi C
Istituto di Ricerca per l'Elettromagnetismo e i Componenti Elettronici Consiglio Nazionale delle Ricerche, Napoli, Italy.
Biochem Biophys Res Commun. 1991 Apr 15;176(1):194-200. doi: 10.1016/0006-291x(91)90908-p.
The cytokinesis block micronucleus method, a very sensitive cytogenetic assay, was used to ascertain the possible genotoxic effects of extremely low frequency pulsed magnetic fields in phytohemagglutinin-stimulated human lymphocytes cultures from 16 healthy donors. Four conditions were studied: i) lymphocytes not exposed to the field (control cultures); ii) lymphocytes exposed to the field; iii) lymphocytes treated with mitomycin-C and not exposed to the field; iv) lymphocytes treated with mitomycin-C and exposed to the field. Mitomycin-C-treated cultures were used as control for the micronucleus method, because it is known that mitomycin-C is a potent genotoxic agent, capable of inducing micronuclei. The frequency of micronuclei in field-exposed cultures was similar to the spontaneous frequency observed in control unexposed-cultures. Moreover, the exposure to pulsed magnetic fields did not affect the frequency of micronuclei induced by mitomycin-C, suggesting that, in the experimental conditions used, this kind of field neither affected the integrity of chromosomes nor interfered with the genotoxic activity of mitomycin-C.
胞质分裂阻滞微核法是一种非常敏感的细胞遗传学检测方法，用于确定极低频脉冲磁场对来自16名健康供体的植物血凝素刺激的人淋巴细胞培养物可能产生的遗传毒性作用。研究了四种情况：i）未暴露于磁场的淋巴细胞（对照培养物）；ii）暴露于磁场的淋巴细胞；iii）用丝裂霉素-C处理且未暴露于磁场的淋巴细胞；iv）用丝裂霉素-C处理且暴露于磁场的淋巴细胞。用丝裂霉素-C处理的培养物用作微核法的对照，因为已知丝裂霉素-C是一种强效遗传毒性剂，能够诱导微核。暴露于磁场的培养物中的微核频率与未暴露的对照培养物中观察到的自发频率相似。此外，暴露于脉冲磁场并未影响丝裂霉素-C诱导的微核频率，这表明在所用的实验条件下，这种磁场既不影响染色体的完整性，也不干扰丝裂霉素-C的遗传毒性活性。
