Yamanaka Masahito, Kawano Shogo, Fujita Katsumasa, Smith Nicholas I, Kawata Satoshi
Osaka University, Department of Frontier Biosciences, 2-1 Yamadaoka, Suita, Osaka 565-871, Japan.
J Biomed Opt. 2008 Sep-Oct;13(5):050507. doi: 10.1117/1.2992595.
We demonstrate high-resolution fluorescence imaging in biological samples by saturated excitation (SAX) microscopy. In this technique, we saturate the population of fluorescence molecules at the excited state with high excitation intensity to induce strong nonlinear fluorescence responses in the center of laser focus, which contributes the improvement of the spatial resolution in three dimensions. Using SAX microscopy, we observed stained microtubules in HeLa cells with improved spatial resolution. We also measured the relation of the fluorescence and excitation intensity with several kinds of fluorescence dyes and, in the results, confirmed that SAX microscopy has the potential to observe any kind of fluorescence samples in current usage.
我们通过饱和激发（SAX）显微镜展示了生物样品中的高分辨率荧光成像。在这项技术中，我们用高激发强度使处于激发态的荧光分子群体饱和，以在激光焦点中心诱导强烈的非线性荧光响应，这有助于提高三维空间分辨率。使用SAX显微镜，我们以更高的空间分辨率观察了HeLa细胞中染色的微管。我们还用几种荧光染料测量了荧光与激发强度的关系，结果证实SAX显微镜有潜力观察当前使用的任何种类的荧光样品。
